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Optimized tools for high-confidence gene editing
The Dharmacon Edit-R CRISPR-Cas9 platform provides predesigned, ready-to-use DNA and RNA components to enable rapid and highly functional gene editing experiments.
Place a custom order, or design and order your own crRNA or lentiviral sgRNA with our easy-to-use interface.
Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Simply search for your gene!
The Edit-R trans-activating CRISPR RNA (tracrRNA) is a chemically synthesized and HPLC-purified long RNA required for use with synthetic crRNA to form the complex that programs Cas9 nuclease.
Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.
Pre-defined collections of popular gene families and pathways for high-throughput applications.
Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest.
Algorithm-optimized sgRNA for genome-wide coverage of human, mouse, or rat genes. Provided as high-titer lentiviral particles.
Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.
High-titer pooled screening libraries for pre-defined gene sets in human and mouse.
Arrayed collections of lentiviral sgRNA libraries for high-throughput knockout screening across entire human gene families.
Non-lentiviral vectors provided as endotoxin-free purified DNA for direct co-transfection with Edit-R synthetic crRNA and tracrRNA.
Purified lentiviral particles or plasmid DNA for generation of stable Cas9 nuclease-expressing cell populations. Constitutive or inducible promoter options are available.
Purified Cas9 mRNA for transient Cas9 Nuclease expression.
Purified Cas9 protein ready to use for DNA-free nuclease expression.
Optimized tools for high-confidence genome engineering
The oligos that program Cas9 nuclease to cut at a specific genomic location
Configure the optimal promoter for your cell type to ensure robust expression of Cas9 nuclease
Proper controls are essential to assessment of CRISPR-Cas9 genomic editing experiments
Pooled sgRNA or arrayed crRNA for high-throughput gene editing studies
Design your own crRNA or sgRNA with our easy-to-use interface.
Use this tool to design and order a single-stranded DNA donor (≤ 150 nt) for precise CRISPR-Cas9 gene editing with the HDR pathway.
CRISPR-Cas9 systems can be used with custom RNA guides for several gene editing applications