Previously added items:
Optimized tools for high-confidence gene editing
The Dharmacon Edit-R CRISPR-Cas9 platform provides predesigned, ready-to-use DNA and RNA components to enable rapid and highly functional gene editing experiments.
Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!
Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.
Custom synthetic 100-mer single guide RNA, input your own design or use our flexible design tools
Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.
Pre-defined collections of popular gene families and pathways for high-throughput applications.
Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest.
Algorithm-optimized sgRNA for genome-wide coverage of human, mouse, or rat genes. Provided as high-titer lentiviral particles.
Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.
High-titer pooled screening libraries for pre-defined gene sets in human and mouse.
Arrayed collections of lentiviral sgRNA libraries for high-throughput knockout screening across entire human gene families.
Non-lentiviral vectors provided as endotoxin-free purified DNA for direct co-transfection with Edit-R synthetic crRNA and tracrRNA.
Purified lentiviral particles or plasmid DNA for generation of stable Cas9 nuclease-expressing cell populations. Constitutive or inducible promoter options are available.
Purified Cas9 mRNA for transient Cas9 Nuclease expression.
Purified Cas9 protein ready to use for DNA-free nuclease expression.
Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.
Design and order a single-strand DNA donor (≤ 150 nt) for insertion, deletion, or other alteration.
Design and order a plasmid DNA donor kit for insertion of an mKate2 or EGFP fluorescent marker, or a custom insert
Rapidly and easily assemble a plasmid donor for HDR
Quickly and efficiently build a HDR donor plasmid
PCR components for Edit-R Plasmid Donor Kits
CRISPR-Cas9 systems can be used with custom RNA guides for several gene editing applications
CRISPR-Cas9 genome editing with a synthetic 99-mer single guide RNA
Development of an algorithm for functional knockout, not just cutting
Rigorous alignment tools ensure high specificity of gene editing
An inducible lentiviral Cas9 nuclease for increased experimental flexibility
Considerations for successful HDR experimental design