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    SMARTchoice Platform

    Maximize expression with your choice of constitutive and inducible promoters!

    Flexibility to customize your vector constructs with your choice of:

    • seven constitutive promoters
    • four inducible promoters
    • two fluorescent reporters, or no reporter at all!

    The SMARTchoice platform gives you a choice of promoters and reporters for maximum experimental flexibility

    Choose your promoter!

    In expression vectors, promoter activity differs between cell types. With SMARTchoice vectors, you can choose the promoter with the highest activity in your cell type. This is the best way to maximize expression, and thereby improve your results.

    If you are uncertain of relative promoter activity in your cells, the Dharmacon SMARTchoice Promoter Selection Plate and the SMARTchoice Inducible Non-targeting Control 4-pack allow straightforward identification of the optimal promoter in your cells of interest.

    Choose your reporter!

    Fluorescent reporters help with visual tracking of transduction and confirmation of expression. SMARTchoice vectors give you the option of TurboGFP or TurboRFP for fluorescent reporting. For some experiments, a fluorescent reporter may be undesirable and for those instances, you can select "no fluorescent reporter" as a vector option.

    SMARTchoice Promoter Selection Tools Promoters Order Now
    SMARTchoice shRNA Promoter Selection Plate Evaluate 7 Order
    SMARTchoice Inducible Non-targeting Control 4-Pack Evaluate 4 Order
    SMARTvector Lentiviral shRNA and shMIMIC microRNA Controls
    SMARTvector Non-targeting shRNA Control 7 choices Order
    SMARTvector Human, Mouse and Rat GAPD shRNA Controls 7 choices
    SMARTvector Human, Mouse and Rat PPIB shRNA Controls hCMV only
    SMARTvector Inducible Lentiviral shRNA and shMIMIC microRNA Controls
    SMARTvector Inducible Non-targeting shRNA Control 4 choices Order
    SMARTvector Inducible Human, Mouse and Rat GAPD shRNA Controls 4 choices
    SMARTvector Inducible Human, Mouse and Rat PPIB shRNA Controls 4 choices

    SMARTchoice options are available on these products

    Constitutive Workflow

    Following optimization of basic transduction conditions, transduce cells using the SMARTchoice Promoter Selection Plate to assess the activity of seven different promoters.

    Next, order shMIMIC lentiviral microRNAs or SMARTvector Lentiviral shRNAs of interest with your selected promoter and reporter for maximized experimental success. Don’t forget to order the matched positive and negative controls!

    Inducible Workflow

    Start by optimizing basic lentiviral transduction conditions such as optimal cell density and polybrene concentration. Following optimization, transduce cells using the SMARTchoice Inducible Non-targeting control four-pack and evaluate the cell-specific activity of the four different inducible promoters. Next, order shMIMIC Inducible Lentiviral microRNAs or SMARTvector Inducible Lentiviral shRNAs of interest with your selected promoter and reporter for maximized experimental success. Don’t forget to order the matched positive and negative controls!

    SMARTchoice Resources

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