Human Cystic Fibrosis EST cDNA


EST cDNA libraries from human lung tissue, cystic fibrosis (CF), and non-CF epithelia.

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Human Cystic Fibrosis EST cDNA Collection
EHS1020 glycerol stock - Inquireselect
Human Cystic Fibrosis EST cDNA clone
EHS1019 glycerol stock - $84.00select

Cystic fibrosis (CF) is a recessive genetic disorder affecting the lungs and is characterized by abnormalities in the epithelia that lead to inflammation and infection. In an effort to define the transcript profile of airway epithelia, researchers at the University of Iowa have created and sequenced cDNA libraries from human lung tissue as well as cystic fibrosis (CF) and non-CF epithelia.

Their efforts produced > 50,000 3'-expressed sequence tags (3'-ESTs), including thousands of transcripts not previously identified as being expressed in the lung. In addition to enabling large-scale expression profiling, the Human Cystic Fibrosis EST cDNA collection has additional applications for studies of pulmonary diseases, including cystic fibrosis, respiratory infections, and asthma.

Highlights

  • Contains ~53,000 3'-expressed sequence tags (3'-ESTs), 6416 of which were not previously identified in the lung
  • This set also includes 879 novel 3'-ESTs that were not previously represented in the National Center for Biotechnology Information (NCBI)
  • Available as individual clones or as an entire collection
  • All clones are in the pT7T3-Pac vector; host is DH10B

Why full-insert sequencing is important

  1. EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
  2. Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
  3. Because these EST clones are not fully sequenced, we cannot guarantee clone content.

 
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Reference:
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.

Disclaimer

GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (ts.dharmacon@ge.com)

  
HazardousNo
Shipping ConditionAmbient,Dry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C

Individual Clones

Clones are provided as ready-to-use live culture in 2 mL tubes. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Clones ship at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Clone Orders

Orders of 50 clones or greater are provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

References

  1. T. E. Scheetz et al., Large-scale gene discovery in human airway epithelia revelas novel transcripts. Physiological Genomics. 17(1), 69-77 (12 March 2004).

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