Human Eye EST cDNA Collection


The Human Eye EST cDNA Collection contains a large number of expressed sequence tag (EST) cDNA clones derived from human eye tissue.

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Human Eye cDNA EST cDNA clone
EHS1031 glycerol stock - $84.00select
Researchers at the University of Iowa have created a unique resource for research of the human visual system. Containing more than 100,000 expressed sequence tags (ESTs), the Human Eye EST cDNA Collection contains cDNAs derived from tissue types, including RPE and choroid, foveal and macular retina, whole retina, optic nerve, anterior segment, lens, and fetal eye.

Highlights

  • > 100,000 EST clones represent appoximately 15,000 genes that are expressed in the human eye
  • Multiple types of eye tissue are represented in the collection
  • All clones in this collection were constructed using the pT7T3D-Pac vector with a modified polylinker and are ampicillin resistant
  • The host used in construction was E. coli DH10B

Why full-insert sequencing is important

  1. EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
  2. Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
  3. Because these EST clones are not fully sequenced, we cannot guarantee clone content.

 
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Reference:
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.

  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C

Individual Clones

Clones are provided as ready-to-use live culture in 2 mL tubes. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 4 to 6 days of receiving your order, we will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Clone Orders

Orders of 50 clones or greater are provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

References

  1. T. E. Scheetz et al., Large-scale gene discovery in human airway epithelia revelas novel transcripts. Physiological Genomics. 17(1), 69-77 (12 March 2004).

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