Mouse BMAP EST cDNAs are distributed as part of the Brain Molecular Anatomy Project (BMAP), a trans-NIH project aimed at understanding gene expression and function in the nervous system. One of the major goals of BMAP is to discover and catalog all genes expressed in the nervous system under both normal and abnormal conditions.
Please visit the NIH BMAP website for more information pertaining to BMAP resources, related courses, and scientific meetings, reports and publications, funding opportunities, and major contacts.
Arrayed cDNA libraries represent 10 regions of adult mouse brain, spinal cord, and retina
Over 50,000 EST clones sequenced and clustered, resulting in more than 37,000 unique clones arrayed as the BMAP Non-redundant Collection
Clones are available individually or as a complete library collection
BMAP has two major scientific goals:
- Gene discovery: to catalog of all the genes expressed in the nervous system, under both normal and abnormal conditions.
- Gene expression analysis: to monitor gene expression patterns in the nervous system as a function of cell type, anatomical location, developmental stage, and physiological state, and thus gain insight into gene function.
Why full-insert sequencing is important
EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
Because these EST clones are not fully sequenced, we cannot guarantee clone content.
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (firstname.lastname@example.org)