Mouse NIA EST cDNAs


Pursue gene expression studies to better understand the biology of aging. Choose from over 22,000 mouse EST cDNA clones provided by the National Institute of Aging (NIA).

Enter a common gene identifier and click SEARCH to find products for your gene.

 
NIA Mouse 15K EST cDNA Collection
EMM3837 glycerol stock - Inquireselect
NIA Mouse EST cDNA
EMM3836 glycerol stock - $99.00select
Mouse NIA EST cDNAs are provided as part of the National Institute on Aging’s (NIA) biological resources.  The NIA, one of the 27 Institutes and Centers of NIH, leads a broad scientific effort to understand the nature of aging and to extend the healthy, active years of life. NIA is the primary Federal agency supporting and conducting Alzheimer's disease research.

Highlights

Over 22,000 mouse EST clones have been made available in two separate releases: a 15K set and a 7.4K set.

NIA 15K Mouse cDNA Clone Set

  • Made up of ~15,000 "unique" cDNA clones selected from among 52,374 ESTs
  • Derived from pre- and peri-implantation embryos, E12.5 female gonad/mesonephros, and newborn ovary
  • Up to 50% of clones are derived from novel genes
  • Average insert size of 1.5 kb

NIA 7.4K Mouse cDNA Clone Set

  • Made up of ~7,400 cDNA clones with no redundancy within the set or with NIA Mouse 15K set
  • Average insert sizes range from 1.5 kb for short insert clones to 2.5-3.0 kb for long-insert enriched clones

Please visit the NIA website for comprehensive information about the collections as well as the individual clones.

Why full-insert sequencing is important

  1. EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
  2. Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
  3. Because these EST clones are not fully sequenced, we cannot guarantee clone content.

 
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Reference:
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.

Disclaimer

GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (ts.dharmacon@ge.com)

  
HazardousNo
Shipping ConditionAmbient,Dry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.

Bulk Clone Orders

Orders of the full collection or of 50 clones or greater will be provided as live cultures in 96-well microtiter plates. Each well contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.

Product and Technical Manuals