Mouse NIA EST cDNAs are provided as part of the National Institute on Aging’s (NIA) biological resources. The NIA, one of the 27 Institutes and Centers of NIH, leads a broad scientific effort to understand the nature of aging and to extend the healthy, active years of life. NIA is the primary Federal agency supporting and conducting Alzheimer's disease research.
Over 22,000 mouse EST clones have been made available in two separate releases: a 15K set and a 7.4K set.
NIA 15K Mouse cDNA Clone Set
Made up of ~15,000 "unique" cDNA clones selected from among 52,374 ESTs
Derived from pre- and peri-implantation embryos, E12.5 female gonad/mesonephros, and newborn ovary
Up to 50% of clones are derived from novel genes
Average insert size of 1.5 kb
NIA 7.4K Mouse cDNA Clone Set
Made up of ~7,400 cDNA clones with no redundancy within the set or with NIA Mouse 15K set
Average insert sizes range from 1.5 kb for short insert clones to 2.5-3.0 kb for long-insert enriched clones
Please visit the NIA website for comprehensive information about the collections as well as the individual clones.
Why full-insert sequencing is important
EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
Because these EST clones are not fully sequenced, we cannot guarantee clone content.
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (firstname.lastname@example.org)