The Mouse Organ of Corti EST cDNA collection was constructed by the National Institute on Deafness and Other Communication Disorders (NIDCD) in order to study the transcriptional repertoire of the organ of Corti during the onset of hearing. A database of ESTs was generated from in-depth sequencing of a non-amplified mouse organ of Corti cDNA library. This resource provides both a view of the profile of gene expression in the OC at the onset of hearing and a tool to identify novel genes of importance in hearing.
Dissected from post-natal through P13 developmental stages of mouse organ of Corti tissue
Represents ~26,000 EST clones offered individually or as a collection
Directionally cloned into the pBluescript SK - vector using the XhoI (3’) and EcoRI (5’) restriction sites
Why full-insert sequencing is important
EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
Because these EST clones are not fully sequenced, we cannot guarantee clone content.
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (firstname.lastname@example.org)
Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.
Bulk Clone Orders
Orders of the full collection or of 50 clones or greater will be provided as live cultures in 96-well microtiter plates. Each well contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.
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