NEI expressed sequence tag (EST) and cDNA clones are distributed from the collection of Graeme Wistow and Katherine Peterson of the Section of Molecular Structure and Function at the National Eye Institute (NEI) who have overseen the generation of cDNA clones from various regions of the mammalian eye.
NEIBank is a database of assembled EST data from eye tissue libraries.
- EST cDNAs from the eye of human, mouse, rabbit, and dog offered as individual clones
NEIBank libraries have the highest level of curation and quality
Additional information regarding these cDNA clone libraries is available on the project website at http://neibank.nei.nih.gov.
Why full-insert sequencing is important
EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
Because these EST clones are not fully sequenced, we cannot guarantee clone content.
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (firstname.lastname@example.org)