University of Iowa EST cDNA


The University of Iowa Rat EST cDNA Clone collection contains over 150,000 rat Expressed Sequence Tag (EST) clones generated from adult and embryonic rat tissues.

Enter a common gene identifier and click SEARCH to find products for your gene.

 
Non-redundant Rat EST cDNA
ERN1017 glycerol stock - $99.00select
University of Iowa Human Placenta EST cDNA clone
EHS1194 glycerol stock - $99.00select
Non-redundant Rat EST cDNA Collection
ERN1016 glycerol stock - Inquireselect
University of Iowa Rat EST cDNA
ERN1184 glycerol stock - $68.00select

Under "A Program for Rat Gene Discovery and Mapping" supported by the National Heart, Lung and Blood Institute and the National Eye Institute, University of Iowa researchers have developed over 80 normalized and subtracted cDNA libraries. ESTs were generated from the 5′ end for a nonredundant set of cDNAs, and every EST (3′ and 5′) generated during this project that was uncontaminated and of sufficient quality was annotated with library information and other identified features, and submitted to the dbEST database at the NCBI.

ESTs were generated from the 5′ end for a nonredundant set of cDNAs, leadiner to over 30,000 unique clones being selected and arrayed into the Rat Non-redundant Collection, sequence verified by the University of Iowa.

Highlights

  • EST cDNAs were derived from over 100 different adult and embryonic tissues from the Rattus norvegicus strain Sprague-Dawley
  • Over 30,000 5'-ESTs were selected and arrayed into the Rat Non-redundant Collection
  • Libraries were constructed using the pT7T3D-Pac vector with a modified polylinker and provide ampicillin resistance. Host used in construction was E. coli DH10B

Why full-insert sequencing is important

  1. EST (expressed sequence tag) clones are not all fully sequenced, only a portion of the cDNA has been sequenced.
  2. Without full-insert sequencing, it is impossible to know whether a clone contains SNPs, alternative splice variants, insertions, or deletions.
  3. Because these EST clones are not fully sequenced, we cannot guarantee clone content.

 
fl-04
 
Reference:
Baross A et al systematic recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.

Disclaimer

GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (ts.dharmacon@ge.com)

  
HazardousNo
Shelf Life12 Months
Shipping ConditionAmbient,Dry Ice
Storage Condition-80 C

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.

Bulk Clone Orders

Orders of the full collection or of 50 clones or greater will be provided as live cultures in 96-well microtiter plates. Each well contains LB medium supplemented with 8% glycerol and the appropriate antibiotic.

References

  1. T. E. Scheetz et al.High-throughput gene discovery in the rat. Genome Res. 14(4), 733-741 (April 2004).
  2. T. E. Scheetz et al.Generation of a high-density rat EST map. Genome Res. 11(3), 497-502 (March 2001).

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