Expression Ready MGC cDNA Libraries


Human Expression Ready MGC cDNA Libraries are available as pre-defined gene families of clones in pCMV-SPORT6 expression vectors.
Expression Ready MGC cDNA Libraries are fully sequenced human cDNA arrayed sets from the Mammalian Gene Collection (MGC) that are derived from genes falling under specific Gene Ontology terms and are present in pCMV SPORT6 expression vectors.

Highlights

  • Insert is fully sequenced and guaranteed to match corresponding BC Accession Number
  • Expression-ready vectors eliminate additional cloning steps
  • Robust CMV promoters drive cDNA expression
  • Gateway sites flanking coding sequence allow for additional flexibility
  • Convenient predefined library format

MGC cDNA gene family libraries are also available in additional vectors.

Custom cDNA Libraries

Custom libraries are available. Please email requests to Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.

Disclaimer

GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (ts.dharmacon@ge.com)

  
HazardousNo
Shipping ConditionDry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C
Libraries will be provided in 96-well microtiter plates. These will ship via overnight delivery and should be stored at –80°C immediately upon receipt.
Human Expression Ready cDNAs - pCMV SPORT6 vector map

Human Expression Ready cDNAs - pCMV SPORT6 vector map

Human Expression Ready cDNAs - pCMV SPORT6 vector map

The pCMV SPORT6 vector is driven by a CMV promoter and includes Gateway recombination sites, 5' and 3' multiple cloning sites, and an Ampicillin resistance marker.


References

  1. MGC Program Team, Generation and initial analysis of more than 15,000 Full-length Human and Mouse cDNA Sequences.PNAS USA.99(26), 16899-16903 (24 December 2002).
  2. MGC Project Team, The status, quality, and expansion of the NIH Full-length cDNA Project: The Mammalian Gene Collection (MGC).Genome Res.14(10B), 2121-2127 (October 2004).
  3. MGC Project Team, The completion of the Mammalian Gene Collection (MGC).Genome Res.19(12), 2324-2333 (December 2009).

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