The Center for Cancer Systems Biology of the Dana-Farber Institute has created a collection of human open reading frames (ORFs) cloned into a Gateway-entry vector, known as the CCSB Human ORFeome Collection. The Gateway-adapted ORFs are ideal for easily moving gene content into compatible destination vectors for various proteomics studies. The native stop codons have been removed from the ORFs for maximum flexibility.
This collection, also known as hORFeome v5.1, contains over 15,000 individual clones representing more than 12,000 unique genes. These clones are derived from fully sequenced Mammalian Gene Collection (MGC) full-length cDNAs and have been subsequently cloned into a Gateway recombinational entry vector. Each coding sequence (CDS) was amplified for only 25 cycles with gene-specific primers and high-fidelity polymerase, minimizing the risk of PCR-induced mutations.
Genome-scale coverage - over 12,000 human genes available as ORF clones
Gateway entry vectors facilitate convenient transfer of ORFs into compatible prokaryotic, mammalian, viral, or insect expression systems
Native stop codons have been removed to allow for expression of tagged fusion proteins
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (firstname.lastname@example.org)
Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order, we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.
Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
- J. R. Rual et al., Human ORFeome Version 1.1: a platform for reverse proteomics. Genome Research.14, 2128–2135 (2004).
- P. Lamesch et al., hORFeome 3.1: a resource of human open reading frames covering over 10,000 human genes. Genomics.89, 307-315 (2007).
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