The Human ORFeome v8.1 Collection represents the most recent version of the Human ORFeome from the Center for Cancer Systems Biology at Dana-Farber Cancer Institute (CCSB-DFCI). hORFeome v8.1 Collection has undergone more extensive QC than previous versions, having had clonal isolates verified by next-gen sequencing.
Altogether, this collection represents almost 12,000 unique genes. Originally derived from fully-sequenced MGC cDNA clones, these fully sequenced* ORFs have been Gateway-adapted to enable efficient transfer of the ORF construct into compatible expression vectors.
Fully-sequenced human ORF collection
Gateway-entry format for efficient recombination
Stop codons have been removed to allow for fusion proteins
GE Healthcare Dharmacon (“Dharmacon”) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support.
More information on the creation of this library can be found in the following publication: X. Yang, et al., A public genome-scale lentiviral expression library of human ORFs. Nat Methods 8(8), 659-661 (2011).