ORFeome Collaboration Library


The ORFeome Collaboration Libraries contain fully sequenced, genome-scale human and mouse open reading frame (ORF) libraries cloned into Gateway-entry vectors.
The ORFeome Collaboration (OC) is a group of academic and commercial laboratories formed to develop an unrestricted source of sequence-validated human ORF clones. Using MGC cDNA clones as starting material, the OC has been able to create over 10,000 full-length human ORF clones, promoting the goal of providing at least one ORF for each of the ~18,500 defined human genes. Dharmacon is a major distributor for the OC, providing individual clones, gene family libraries, and whole-genome collections to academic and commercial customers.

Using Gateway recombinational cloning system in all of the OC ORF clones allows for efficient transfer of the ORFs from one vector to another, providing a broad choice of destination vectors. OC ORFs are available with or without native stop codons, providing options for native and fusion-ready protein expression.

Highlights

  • Fully-sequenced, genome-scale ORF collection
  • Easily transferable into any Gateway-adapted expression vector
  • Available with and without stop codons
ORFeome Collaboration - Serving the research community with Human ORF clones

Disclaimer

Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support (ts.dharmacon@ge.com)

  
HazardousNo
Shipping ConditionDry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C
Libraries are provided as glycerol stocks in 96-well microtiter plates. These ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on library orders.

References

  1. J. F. Rual et al., Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 14, 2128-2135 (2004).
  2. P. Lamesch et al., hORFeome v3.1: A Resource of Human Open Reading Frames Covering over 10,000 Human Genes. Genomics. 89(3), 307-315 (Epub 5 January 2007, March 2007). [In preparation]

Product and Technical Manuals

Safety Data Sheets