Saccharomyces cerevisiae is a powerful model for understanding genetic regulation in eukaryotic cells. Dharmacon yeast resources include multiple collections for interrogating the yeast genome, including Tagged ORFs, Kockout Strains, Protein-Protein Interaction Collections and Mutant Strains and Screening Collections.
The Yeast ORF collection consists of Gateway-adapted yeast ORFs cloned into expression plasmids. ORFs can be expressed as fusion proteins or moved to other vectors.
The Yeast Kinase YFP Fusion collection contains a number of kinase genes that have been cloned with a C-terminal YFP tag and promoter region into a Gatewa-entry vector.
Yeast-TAP Tagged ORF library allows the purification and the selection of the yeast proteome and associated components using two affinity selection steps in tandem.
The Molecular Barcoded Yeast (MoBY) ORF library was designed to identify genes in Saccharomyces cerevisiae that confer drug resistance when mutated, providing a tool for identifying drug-resistant mutations in yeast.
The Yeast GST-tagged ORFs are a a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.
The Yeast HA-Tagged Collection contains mutagenized yeast strains with triple hemagglutinin (HA) epitope tags, useful for immunolocalization, immunoprecipitation, and analysis of binding sites.
The Yeast Barcoder Collection is a set of approximately 1,400 donor strains with unique DNA sequence tags (barcodes) that can be systematically transferred to any S. cerevisiae collection.
The Yeast Knockout (YKO) Collection contains over 6,000 gene-disruption mutants as a unique tool for the functional analysis of the yeast genome.
The Yeast Tet-Promoters Hughes Collection (yTHC) with 800 essential yeast genes under control of a tetracycline-regulated promoter permits experimental regulation of essential genes.
Parental yeast strains for use with the Yeast Knockout (YKO) and other yeast collections.
Protein-Protein Interaction Collections
The Yeast Protein Interactome Collection allows for genome-wide in vivo screening of protein-protein interactions (PPI) in Saccharomyces cerevisiae.
The Yeast Cross and Capture system is designed to examine protein-protein interactions and protein modifications using 6xHIS and VSV tagged haploid yeast strains.
Mutant Strains and Screening Collections
The Yeast Insertional Mutant collection contains a set of over 3,600 gene disruption mutants for phenotypic analysis.
Systematic mutations have been engineered into the Yeast Synthetic Histone H3 and H4 mutants in order to elucidate nucleosome functionality.
Designed to facilitate comprehensive overexpression screens, the Yeast Genomic Tiling Collection contains the yeast (Saccharomyces cerevisiae) genome in a 2 micron-based LEU2 vector.
Reduce mRNA levels of essential yeast genes by four- to ten-fold with the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library.
Non-Mammalian cDNAs and ORFs