The Andrews lab at the University of Toronto has developed a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.
More than 80% of the S. cerevisiae genome is represented. ORFs are plasmid-encoded, tagged at the N-terminus with GST, and expressed under control of the GAL1/10 promoter (Zhu et al, 2001). The Yeast GST-tagged ORFs is in a BY4741 (MATa) background and has been used for genome-scale overexpression screening (Sopko et al, 2006).
More than 5,000 yeast strains overexpress an ORF through galactose induction
Collection represents more than 80% of the S. cerevisiae genome
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