Cherry-pick Clone Libraries
Choose from our predesigned product lines of shRNA and over-expression reagents to build your own custom library.
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Cherry-pick Clone Libraries

Enjoy fast and easy online ordering of vector-based reagents – no quote required!

Choose from our predesigned products lines of shRNA and over-expression reagents to build your own custom library.

  • Our online Library Plater offers the flexibility to select clones and generate a library for your unique experimental needs
  • Choose vector-based reagents from any of the glycerol stock formatted Dharmacon shRNA product lines OR mammalian gene-expression collections. For synthetic reagents, please use Cherry-pick Libraries
  • Select specific clones for a gene or add everything available
  • Clones will automatically be separated onto different plates by antibiotic resistance marker and product format

Overview

When Dharmacon pre-defined shRNA or over-expression libraries do not match your experimental requirements, you can make your own custom clone library with ease, speed and economy.

You are able to cherry-pick from our glycerol stock format shRNA and mammalian over-expression product lines for human and mouse reagents to create the ideal clone collection for your particular needs.

Here's how:

cherry-pick clone libraries workflow
  1. Load gene identifiers for your desired products
    • NCBI Gene ID or Gene Symbol
    • Dharmacon product catalog numbers
  2. Select the product type
  3. Review the results for accuracy and product availability
  4. Select the product lines and product formats to include on the plate
  5. Add to cart and check out!
 
 

Cherry-pick Library options

Use the Library Plater to order these products in 96-well plates
shRNA Product Lines GIPZ, TRIPZ, TRC
Over-expression Product Lines Mammalian Genome Collection (MGC), Precision LentiORFs, CCSB-Broad Lentiviral Expression Library, ORFeome Collaboration Collection, Human ORFeome v8.1 Library, CCSB Human ORFeome
Product format Glycerol stock
Species Human or mouse
Plate type 96 well; 20 well minimum of same product line

Product Line Selection

Which shRNA product is right for you?

Dharmacon offers three complete predesigned product lines across human and mouse genomes. Use the table below to help you in determining the right shRNA product for your needs.

  GIPZ TRIPZ TRC
Species Human, mouse Human Human, mouse
Promoter Human CMV (Pol II) TRE min-CMV U6 (Pol II)
Hairpin format micorRNA-adapted micorRNA-adapted Simple
Fluorescent reporter TurboGFP TurboRFP None
Inducible expression No Yes No

Which over-expression product is right for you?

Dharmacon offers five different mammalian open reading frame (ORF) collections and the Mammalian Genome Collection (MGC) cDNA collection. Use the table below to help you in determining the right over-expression product for your needs.

  Mammalian Genome Collection (MGC) Precision LentiORF CCSB-Broad Lentiviral Expression ORF ORFeome Collaboration Collection Human ORFeome V8.1 CCSB Human ORFeome
Format cDNA ORF ORF ORF ORF ORF
Species Human, Mouse Human Human Human, Mouse Human Human
Fully Sequenced Yes Yes Yes Yes Yes No
Expression ready Some Yes Yes No No No
Subcloning method Restriction digest N/A N/A Gateway® Gateway® Gateway®
Selection marker N/A GFP V5 epitope tag N/A N/A N/A

Product Formats Available for Cherry-pick Library Plater

All products are delivered as arrayed glycerol stocks in 96-well plates

shRNA clones

Select from either: GIPZ, TRIPZ, or TRC lentiviral shRNA collections; minimum 20 constructs per order. We recommend selecting 3-5 constructs per gene.

Over-expression clones

MGC collection: Cannot be mixed with other collections, minimum 20 constructs per order; clones will be plated according to antibiotic requirements

ORF collections

May be combined with other ORF collections to increase coverage for desired library; minimum 20 constructs from a single collection per order. Clones will be plated by collection. (e.g. a customer library of 20 ORFeome collaboration clones and 5 hORFeome v8.1 clones will qualify for library plating, a custom library of 15 ORFeome collaboration clones and 10 hORFeome v8.1 will not)

 

microRNA-adapted shRNAs

Designed using microRNA scaffold-specific attributes for highly efficient processing via the endogenous RNAi pathway while minimizing off-target potential

Simple hairpin shRNAs

Offers a classic hairpin, rules-based shRNA design utilizing a simple hairpin of 21 base pair sense and antisense stem, and a 6 basepair loop

  • TRC Lentiviral shRNA

    Lentiviral shRNA collection from The RNAi Consortium (TRC) with coverage in human and mouse.

cDNA and ORF Libraries

Pre-defined gene family collections are available for many of our cDNA and ORF products. Choose the product line that best fits your experimental needs.

  • Mammalian Genome Collection

    The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

  • Precision LentiORFs

    Expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

  • CCSB-Broad Lentiviral Expression Library

    Expression-ready human ORFs in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

  • ORFeome Collaboration Libraries

    Sequence-verified human and mouse open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

  • Human ORFeome V8.1 Library

    Fully-sequenced collection of Gateway-adapted ORFs representing the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

  • CCSB Human ORFeome Libraries

    The Gateway® adapted human Entry ORF libraries, ideal for easily moving entire families of ORFs into your choice of protein expression systems.