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    CRISPRa Guide RNA

    Predesigned synthetic and lentiviral reagents for gene activation

    CRISPR activation (CRISPRa) is a powerful method for overexpressing genes for functional analysis. In conjunction with nuclease-deactivated Cas9 (dCas9) fused to activator domains, these guide RNAs offer an efficient method for targeted transcriptional activation.

    Get started with predesigned CRISPRa guide RNA products

    • Edit-R CRISPRa synthetic crRNA

      Offering coverage of the human and mouse genomes, these synthetic RNAs are modified for nuclease resistance and available as pools of four or individual crRNA reagents.

    • Edit-R CRISPRa Lentiviral sgRNA

      Lentiviral particles or glycerol stocks of single guide RNA are predesigned for genome-wide coverage of human and mouse genes in an optimized vector backbone.

     
     

    All Edit-R CRISPRa guide RNAs are predesigned with the algorithm described by Horlbeck et. al[1]., who performed a systematic study of protospacer-adjacent motif (PAM) sites that are adjacent to transcriptional start sites (TSS) to identify features that contribute to CRISPRa activity.

    Guide RNAs are a key component of the CRISPRa system

    In addition to expression of an S. pyogenes catalytically deactivated or dead Cas9 (dCas9) fused to activation domains, such as dCas9-VPR or SunTag-VP64[3], the CRISPR-Cas9 system requires a specific RNA moiety which complexes with the dCas9-VPR and targets a region adjacent to a gene’s transcriptional start site (TSS). These guide RNAs take two forms:

    • A chemically synthesized trans-activating CRISPR RNA (tracrRNA) plus a synthetic CRISPR RNA (crRNA) targeting a region adjacent to the transcriptional start site of the gene of interest (Figure 1a)
    • or
    • An expressed single guide RNA (sgRNA) that consists of both the crRNA and tracrRNA as a single construct with a scaffold that is optimized for high efficiency activation (Figure 1b)

    References

    1. Horlbeck MA, Gilbert LA, et. al. Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255
    2. Chavez A, Scheiman J et. al., Highly efficient Cas9-mediated transcriptional programming Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. 10.1038/nmeth.3312 PubMed 25730490
    3. Tanenbaum ME, Gilbert LA, et. al. A protein tagging system for signal amplification in gene expression and fluorescence imaging. Cell. 2014;159(3):635-646. doi:10.1016/j.cell.2014.09.039
     

    Synthetic Guide RNA

    • Edit-R CRISPRa predesigned crRNA

      Offering coverage of the human and mouse genomes, these synthetic RNAs are modified for nuclease resistance and available as pooled or individual crRNA reagents.

    • Edit-R tracrRNA

      Edit-R trans-activating CRISPR RNA (tracrRNA) is a synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs dCas-VPR. It is modified for nuclease resistance.

    • Edit-R CRISPRa Positive crRNA Controls

      Pooled or individual crRNA controls for assessment of optimal experimental conditions for gene activation

    • Edit-R CRISPRa Non-targeting crRNA Controls

      Non-targeting controls to evaluate baseline cellular responses to CRISPRa components in the absence of gene target-specific crRNA

    • Cherry-pick libraries

      Upload your own gene list to customize and order plates of predesigned crRNA for CRISPRa studies across tens or thousands of genes

    Lentiviral Guide RNA

     

    Resources for CRISPR Activation

     
     

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