• Custom RNA Synthesis  

    Custom Synthesis Overview

    Custom Synthesis Overview

    Custom Single-stranded RNA  
    Single-stranded RNA Synthesis Order
    Multiple Single-stranded RNA Syntheses Order
    Custom siRNA  
    siRNA Synthesis Order
    Modified siRNA Synthesis Order
    Multiple siRNA Syntheses Order
    Custom SMARTpool Design & Synthesis Order
    Custom microRNA  
    Custom microRNA Request Quote
    siDESIGN Center  
    siRNA Design Tool Design Now

    Single-stranded RNA

    Design a single-stranded RNA molecule with a wide variety of chemical modifications, learn about our capabilities for long RNA oligos, dye labeling, custom amidites and more.

    siRNA

    Learn about customizing your siRNA with proprietary modification patterns or other chemical modifications, investigate your options for in vivo RNAi, design your own siRNA using the siDESIGN Center, learn about custom SMARTpool options and more.

    microRNA

    Customize a miRIDIAN Mimic or Hairpin Inhibitor, or design your own microRNA reagent with specialized chemical modifications, in vivo processing, gram quantities and more.

    2'-ACE Chemistry

    RNA applications can be executed with greater ease and confidence by utilizing the powerful advantages of the 2'-ACE technology.

    • Greater reproducibility than any other chemistry
    • Crude yields of unmatched purity without additional purification time and expense
    • Maximum sequence fidelity and integrity due to enhanced coupling efficiency

    Download the Tech Note 2'-ACE RNA Synthesis Chemistry for more information on the details of the RNA synthesis platform.

    All synthetic RNA oligos must be chemically protected at multiple sites during synthesis, regardless of the chemistry platform used. However, 2'-ACE synthesis chemistry allows for the quickest and mildest deprotection conditions compared to other chemistries like 2'-tBDMS and 2'-TOM. Short deprotection times and a mild chemical environment promote the highest level of purity for synthetic RNA in the marketplace today.

    For unmodified, unpurified RNA, the following approximate percentages of full-length material can be expected:

      2'-ACE 2'-tBDMS 2'-TOM
    ASCE* >99% >98% 95%
    21-mer ** 81% 65% 34%
    50-mer ** 61% 36% 8%
    Coupling reaction Fast Slow Slow
    Deprotection conditions Aqueous Organic Organic
    Product can be delivered in 2'-protected or deprotected state Yes No No

    *The average stepwise coupling efficiencies (ASCE) for each method.
    **Percent full-length material listed above are approximate; exact yields will depend on sequence composition.

    What is the difference between scale and yield?

    SCALE

    • Relative amount of starting material packed into an RNA synthesis column
    • Typically expressed as micromole (µmol) increments

    YIELD

    • Final amount of material delivered
    • Typically expressed as nanomole (nmol), micromole (µmol), milligram (mg), or gram (g) amounts

    SCALE ≠ YIELD

    Actual yields obtained for the final product may range from 10%-90% of the scale, depending on the oligo specifications (i.e. modification type, length, purification process, etc.).

    If you have a minimum yield requirement for your experiments, please contact Technical Support for recommendations on which scale to select for your online order.

    Available synthesis scales can accommodate microgram to multi-gram quantities

      Synthesis scales available online Synthesis scales available by request
    siRNA 0.025 µmol - 10 µmol 20 µmol - 2 mmol
    Single-stranded RNA 0.05 µmol - 1 µmol 2 µmol - 2 mmol

    For unmodified siRNA, the following approximate yields can be expected

      Standard (A4)** Purified in vivo in vivo HPLC
    nmol mg nmol mg nmol mg nmol mg
    0.025 µmol scale 20 0.25 - - - - - -
    0.05 µmol scale 40 0.5 20 0.25 - - - -
    0.2 µmol scale 150 2 80 1 100 1.3 50 0.65
    0.4 µmol scale 300 4 160 2 200 2.6 100 1.3
    1.0 µmol scale 750 10 320 4 500 6.6 200 2.6
    2.0 µmol scale 1500 20 640 8.5 1000 13 500 6.6
    5.0 µmol scale 3750 50 1600 21 2505 33 1000 13
    10.0 µmol scale 7500 100 3200 42 5000 66 2500 33

    For single-stranded RNA, yields may vary greatly depending on the specifications of your request (oligo length, sequence, modifications, purification, ect.). Please contact Technical Support for RNA yield estimates.

    *Yields reported above should be considered estimates, and are not guaranteed amounts. Please inquire within if a minimum guaranteed amount is required.
    "**Standard processing includes 2'-ACE deprotection, desalting, and annealing.

    Quality Control

    We pride ourselves on our reputation for high quality RNA synthesis.

    As part of our long-standing commitment to quality, we employ the following standards for quality assurance:

    • All RNA synthesis products (including siRNAs) are analyzed by mass spectrometry and quantified by UV spectroscopy
    • ESI MS for all modified siRNAs and all longer or highly modified RNAs
    • HPLC or PAGE for purification of RNA products
    • UPLC or CGE for quality analysis when appropriate
    • Custom QC methods and documentation are available upon request

    See below for definitions

    Quality control documentation available upon request includes:

    • Mass spectrometry traces
    • UPLC or CGE analysis
    • Duplex or individual strand purity analysis
    • Certificate of Analysis
    • Endotoxin certification
    • Custom QC documentation

    For all mid-scale synthesis projects (200 mg - 10 g), a stringent manufacturing process is adhered to

    • HPLC purification highly recommended to achieve optimal purity
    • Batch records maintained on all synthesis projects (we follow GMP guidelines, but are not GMP certified)
    • Endotoxin testing included on all mid-scale orders
    • Data deliverables include MS, UPLC, endotoxin results, and a Certificate of Analysis

    Definitions:

    ESI MS: Electrospray ionization mass spectrometry
    HPLC: High performance liquid chrmatography
    PAGE: Polyacrylamide gel electrophoresis
    UPLC: Ultra performance liquid chromatography
    CGE: Capillary gel electrophoresis
     

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