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    CRISPR Guide RNA

    Lentiviral and synthetic reagents for targeted gene knockout

    Guide RNAs program Cas9 nucleases to cut at a specific genomic location. The design of an effective, functional guide RNA is critical to achieving specific gene knockout. Take advantage of our algorithm for functional, specific guide RNAs and search our predesigned products for your gene of interest.

    Learn more about guide RNA products

    • Synthetic crRNA

      Pre-designed or custom synthesized for rapid knockout studies across many genes

    • Lentiviral sgRNA

      Pre-designed or custom sgRNA as glycerol stocks and high-titer purified lentiviral particles for small- and large-scale studies.

    All Edit-R predesigned guide RNA (synthetic crRNA and lentiviral sgRNA) are designed with a validated algorithm to improve the likelihood of functional knockout, not just double-strand breaks (DSB). By assessing functional phenotypes for thousands of designs, then validating our design rules in other assay systems, we have established rules for determining target sites that are more likely to give efficient cleavage and functional knockout with high specificity. See the Supporting Data tab for more information.

    Guide RNAs in the CRISPR-Cas9 system

    In addition to expression of the Cas9 nuclease, the CRISPR-Cas9 system requires a specific RNA moiety to recruit and direct the nuclease activity. These guide RNAs take one of two forms:

    • a long, chemically synthesized trans-activating CRISPR RNA (tracrRNA) plus a chemically synthesized CRISPR RNA (crRNA) designed to cleave the gene target site of interest (Figure 1a)
    • -or-
    • an expressed single guide RNA (sgRNA) that consists of both the crRNA and tracrRNA as a single construct (Figure 1b)

    CRISPR Guide RNA Products

    Synthetic Guide RNA

    • Edit-R predesigned crRNA

      Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Simply search for your gene!

    • Edit-R tracrRNA

      The Edit-R trans-activating CRISPR RNA (tracrRNA) is a chemically synthesized and HPLC-purified long RNA required for use with synthetic crRNA to form the complex that programs Cas9 nuclease.

    • Edit-R Synthetic Positive crRNA Controls and Detection Primers

      Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

    • Edit-R Synthetic crRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

    • Edit-R crRNA Libraries

      Plated pre-defined collections of popular gene families for arrayed knockout screening

    • Cherry-pick libraries

      Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest

    Lentiviral Guide RNA

    Custom Guide RNA Ordering & Design Tool

    • CRISPR RNA Configurator

      Place a custom order, or design and order your own crRNA or lentiviral sgRNA with our easy-to-use interface.

    Selection guide

    While the best guide RNA for your experiment may heavily depend on your particular application or cell type, a few basic questions may help to point you in the right direction for product selection.

    All Edit-R predesigned guide RNA (synthetic crRNA and lentiviral sgRNA) are designed with a validated algorithm to improve the likelihood of functional knockout, not just double-strand breaks (DSB).

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