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    Synthetic crRNA

    Pre-designed or custom designs to power rapid, efficient gene editing

    Choose from our predesigned human, mouse, or rat crRNA products to take advantage of our proprietary Edit-R algorithm to improve functionality and specificity, or design your own guide RNA to meet your particular needs.

    • Save time by eliminating cloning and in vitro transcription steps
    • Transfection-ready RNAs for rapid assessment of multiple crRNA per gene
    • Chemically modified for improved nuclease resistance
    • High-quality reagents from Dharmacon 2’ACE chemical synthesis

    Overview

    Edit-R CRISPR-Cas9 synthetic reagents greatly simplify the workflow of permanently knocking out genes by eliminating the time-consuming cloning of individual guide RNA expression vectors. Our novel approach includes predesigned or custom ready-to-use reagents which enable fast assessment of multiple target sites per gene, for multiple genes.

    In contrast to a single guide RNA which is expresses both the crRNA and the tracrRNA as a single chimeric transcript, the Edit-R synthetic crRNA and tracrRNA are transfected together to complex with the Cas9 nuclease to carry out DSB.

    Synthetic crRNA:tracrRNA are compatible with any type of Cas9 nuclease, it is simply a matter of appropriate transfection conditions:

    Cas9 nuclease expression Recommended transfection reagent
    Stably expressed Cas9 nuclease DharmaFECT 1, 2, 3, or 4 for transfection of synthetic crRNA:tracrRNA
    Cas9 nuclease expression plasmid DharmaFECT Duo for co-transfection of plasmid and synthetic crRNA:tracrRNA
    Cas9 mRNA or protein NLS DharmaFECT Duo for co-transfection of mRNA or protein and synthetic crRNA:tracrRNA

    How much crRNA & tracrRNA do I need?

    This table provides the approximate number of experiments that can be carried out for lipid transfection methods at the recommended crRNA:tracrRNA working concentration (25 nM:25nM) in various plate/well formats. Calculations do not account for pipetting errors.
    crRNA
    nmol
    tracrRNA
    nmol
    96-well plate
    100 µL reaction volume
    24-well plate
    500 µL reaction volume
    12-well plate
    1000 µL reaction volume
    6-well plate
    2500 µL reaction volume
    2 2 800 160 80 32
    5 5 2000 400 200 80
    10 10 4000 800 400 160
    20 20 8000 1600 800 320

    Synthetic crRNA Products

    • Edit-R predesigned crRNA

      Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!

    • Edit-R tracrRNA

      Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.

    • Edit-R Synthetic Positive crRNA Controls and Detection Primers

      Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

    • Edit-R Synthetic crRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

    • Edit-R crRNA Libraries

      Plated pre-defined collections of popular gene families for arrayed knockout screening

    • Cherry-pick libraries

      Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest

    Custom Guide RNA Design & Ordering Tools

    • CRISPR RNA Configurator

      Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.

    HDR Donor Template Design & Ordering Tools

    HDR Donor Template Kits

    Workflow

    To facilitate rapid generation of cell lines that constitutively express Cas9 nuclease, the Edit-R Lentiviral Cas9 Nuclease Expression vector is packaged into particles, purified and concentrated for direct transduction. Subsequent transfection of synthetic crRNA and tracrRNA into Cas9-expressing cell lines results in a higher percentage of edited cells in comparison to co-transfection of Cas9 plasmid DNA with synthetic crRNA and tracrRNA without enrichment.

    Supporting Data

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