Gene Editing - HDR Donor Templates

HDR Donor Templates

DNA donor oligos for precise genome modification

Synthetic single-stranded DNA oligonucleotide (ssDNA oligo) donors for precise genomic modification with the homology-directed repair (HDR) pathway.

CRISPR guide RNAs program Cas9 nuclease to cut genomic DNA at a specific location. Once the double-strand break (DSB) occurs, the mammalian cell utilizes endogenous mechanisms to repair the DSB. In the presence of a donor DNA, either a ssDNA oligo or a plasmid donor, the DSB can be repaired precisely using HDR resulting in a desired genomic alteration (insertion, removal, or replacement).

Get started with designing HDR donor templates

Homology-directed repair (HDR) relies on the presence of a donor template with sufficient homology to the regions flanking the cut site. The length of the homology arms and the type of donor (ssDNA oligo or plasmid) will depend on the type and size of precise modification being made. Figure 1 illustrates the use of a donor oligo for insertion of a short DNA region, such as a tag or a restriction site. Go to the Edit-R HDR Donor Designer to see how easy it is to customize a donor oligo!

Donor oligo for precise insertion of a short DNA sequence

donor oligo for precise insertion of a short DNA sequence

For larger insertions, such as a fluorescent marker, a donor plasmid is required, with appropriately longer homology arms to support efficient recombination.

  • Edit-R HDR Donor Designer

    Design and order the donor oligo required for precision insertion, removal or replacement of genomic DNA.

  • CRISPR RNA Configurator

    Place a custom gRNA order, or design and order your own crRNA or lentiviral sgRNA with our easy-to-use interface.