A published, validated siRNA control targeting green fluorescent protein. Functional testing shows effective knockdown at mRNA and protein levels.

In functional testing, the GFP Duplex I control siRNA has demonstrated high efficiency silencing of the green fluorescent protein reporter gene (GFP/eGFP) at both the mRNA and protein levels. 

Because the GFP reporter sequence has been altered by various sources to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match. This siRNA control does not target TurboGFP.

Highlights

  • Target sequence: 5’-GCA AGC TGA CCC TGA AGT TC–3’
  • Appropriate for use in any mammalian cell type
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C

References

  1. N. J. Caplen, S. Parrish, F. Imani, A. Fire, R. A. Morgan, Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A. 98, 9742-9747 (14 August 2001).