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Precision LentiORFs

New gene content now available!


Precision LentiORFs are human cDNA open reading frames (ORFs) cloned into a lentiviral expression vector, used for overexpressing human genes and proteins in mammalian cells
Enter a common gene identifier and click SEARCH to find products for your gene

Common gene identifiers for your gene can be found at NCBI Gene or miRBase.

IdentifierDescriptionExamples
Gene IDThe Gene ID is a unique identifying number for each gene in the RefSeq database and tends to be the most stable gene identifier.
  • 672
  • 22421
Accession NumberAccession Numbers are identifiers for a sequence record. The preferred accession numbers for RNAi products are found in the Reference Sequence (RefSeq) database, a non-redundant, curated, and annotated collection of sequence records for major model organisms. RefSeq accession numbers for mRNA begin with the characters NM_ or XM_ followed by six or nine digits. For gene expression products, the GenBank accession number for the clone can also be used for more specific search results.
  • NM_001798
  • NM_001170537
  • BC068303
Gene SymbolThe Gene Symbol can be the official or alternate symbol as displayed on NCBI Gene.
  • BRCA1
  • CDK1
  • CTNNB1
Gene DescriptionThe Gene Description is also known as the Official Full Name on NCBI Gene.
  • myogenic differentiation 1
  • thyroid peroxidase
  • breast cancer 1, early onset
miRBase IdentifiersmiRBase accession numbers are the most stable identifiers for microRNA. The miRBase ID, or name, may also be used.
  • miRBase ID: hsa-let-7d, mmu-mir-122
  • Precurser Accession: MI0000065, MI0000256
  • Mature Accession: MIMAT0000065, MIMAT0000246

If you require further assistance with your search, please contact Technical Support at ts.dharmacon@ge.com

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ProductCatalog Number 
Precision LentiORF Viral Particle Starter Kit
OHS5836 viral particles kit - $1,364.00select
Precision LentiORF RFP Positive Control
OHS5833 viral particles - $330.00select
Precision LentiORF Human Clone (without native stop codon)
OHS5900 2 x 25 µl viral particles - $758.00select
Precision LentiORF Human Clone (with native stop codon)
OHS5899 2 x 25 µl viral particles - $758.00select
Precision LentiORF Transduction Starter Kit
OHS5835 glycerol kit - $1,818.00select
Precision LentiORF Transfection Starter Kit
OHS5834 glycerol kit - $1,212.00select
Precision LentiORF Human Library - with and without stop codons
OHS6067 1 each - Inquireselect
Precision LentiORF Human Library - with native stop codons
OHS6069 1 each - Inquireselect
Precision LentiORF Human Library - without native stop codons
OHS6068 1 each - Inquireselect
pLOC-TurboRFPa vector map thumbnail image

Click to Enlarge

Vector map of the Precision LentiORF RFP control. For gene-specific constructs, the RFP insert would be replaced by the ORF of interest

Vector map of the Precision LentiORF RFP control. For gene-specific constructs, the RFP insert would be replaced by the ORF of interest

Precision LentiORFs are fully-sequenced human open reading frames (ORFs) that have been cloned into a lentiviral expression vector. Ideal for use in primary or difficult to transfect cells, Precision LentiORFs can be used to create stably expressing cell lines, investigate overexpression phenotypes, or perform RNAi rescue experiments.

Features of the Precision LentiORF backbone include a CMV promoter for robust protein expression, a GFP reporter to visually track protein expression, and blasticidin resistance for selection of stable cell lines. Precision LentiORF clones are available with or without native stop codons, and secondary tags can be added to the MultiTag cloning site located downstream of the coding sequence.

Highlights

  • Lentiviral ORF constructs require no subcloning for mammalian expression
  • Fully sequenced coding sequences
  • GFP reporter is transported to the nucleus for easy visualization of expression
  • Clones are available with or without native stop codons
  • Available as ready-to-transduce viral particles, glycerol stocks, or LentiORF Starter Kits

Applications

Potential applications of Precision LentiORFs include gene overexpression experiments, RNAi rescue (see the RNAi Rescue using Precision LentiORF technical note), and expression of human proteins in mammalian cell lines. Primary and non-dividing cells can be readily transduced with ready-to-use lentiviral particles transfected with Precision LentiORF constructs directly into your cell line. For generating your own viral particles, Trans-Lentiviral ORF packaging kits are also available.

Formats

  • Individual Clones – Glycerol Stock
  • Viral Particles – provided as 2 x 25 µL aliquots with a minimum titer of 10^8 TU/mL
  • LentiORF Starter Kits – Choose from transfection kits, transduction kits, and viral particle kits

Precision LentiORF Viral Particle starter kits include:

  • Two Precision LentiORF constructs provided as 2 x 25µL aliquots of high-titer viral particles (1 x 10^8 TU/mL)
  • Precision LentiORF RFP control provided as 25 µL of high-titer virus

Precision LentiORF Transduction starter kits include:

  • Two Precision LentiORF constructs
  • Trans-Lentiviral packaging mix sufficient for 10 packaging events
  • Calcium Phosphate transfection reagent
  • Precision LentiORF RFP control

Precision LentiORF Transfection starter kits include:

Note

The Precision LentiORF expression vectors are not compatible with third generation packaging systems. We recommend the Trans-Lentiviral Packaging System for use with these vectors.

 

  
HazardousNo
Shelf Life12 Months,13 Months
Shipping ConditionWet Ice,Dry Ice,Ambient
Storage Condition-80 C

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.

Clone Collections

Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Viral Particles

Due to the inherent complexity of high-titer lentiviral particle production, we estimate a 6 to 8 week turnaround time for these orders.

Visually track Precision LentiORF expression

Visually track Precision LentiORF expression

Visually track Precision LentiORF expression

Nuclear localized TurboGFP expressed from cells transduced with Precision LentiORF viral particles.


Cell cycle phenotypes with ORF expressing PLK1

Cell cycle phenotypes with ORF expressing PLK1

Cell cycle phenotypes with ORF expressing PLK1

Immunocytochemistry of HEK293T cells showing cellular morphology of cytoplasm (Anti-tubulin - red) and nucleus (DAPI - blue).


pLOC vector map

pLOC vector map

pLOC vector map

pLOC vector map


Optimize Precision LentiORF expresson level by adjusting MOI

Optimize Precision LentiORF expresson level by adjusting MOI

Optimize Precision LentiORF expresson level by adjusting MOI

Western blot analysis shows that the level of expression can be optimized by controlling the number of viral particles per cell.


References

Citations

  1. E. M. Briso, J. Guinea-Viniegra, L. Bakiri, Z. Rogon, P. Petzelbauer, R. Eils, R. Wolf, M. Rincón, P. Angel, and E. F. Wagner, Inflammation-mediated skin tumorigenesis induced by epidermal c-Fos. Genes & Dev.27:1959-1973 (Sep 2013).
  2. A. V. Shinde, R. K. Motiani, X. Zhang, I. F. Abdullaev, A. P. Adam, J. C. González-Cobos, W. Zhang, K. Matrougui, P. A. Vincent, and M. Trebak, STIM1 Controls Endothelial Barrier Function Independently of Orai1 and Ca2+ Entry. Sci. Signal.6:ra18 (Mar 2013).
  3. H. Fayyad-Kazan, R. Rouas, M. Merimi, N. El Zein, P. Lewalle, F. Jebbawi, M. Mourtada, H. Badran, M. Ezzeddine, B. Salaun, P. Romero, A. Burny, P. Martiat, and B. Badran, Valproate Treatment of Human Cord Blood CD4-positive Effector T Cells Confers on Them the Molecular Profile (MicroRNA Signature and FOXP3 Expression) of Natural Regulatory CD4-positive Cells through Inhibition of Histone Deacetylase. J. Biol. Chem.285:20481-20491 (Jul 2010).