Figure 1. | microRNA Hairpin Inhibitor function for miR-21 miRNA was assayed in HeLa cells, 48 h after transfection using a dual-luciferase reporter system. Each sample was normalized to matching treatment using an empty dual-luciferase reporter.
Figure 2. | Time course of inhibition by three different inhibitor molecule designs targeting endogenous miR-23 in an MCF-7 cell line engineered to stably express a miR 23 dual-luciferase reporter. Inhibitors were transfected using DharmaFECT 1 at 200 nM of the simple mature antisense oligo (AS) or 40 nM of miRIDIAN Hairpin and Competitor E inhibitor designs. Data was normalized to cells with no inhibitor, such that no inhibition is equal to 1.
Figure 3. | Six co-expressed microRNAs (mir-17-5p, miR-18a-5p, miR-19a, miR-20, miR-19b-1 and miR-92-1) from the "Cancer Cluster" were inhibited by miRIDIAN Hairpin Inhibitors. All six miRIDIAN Hairpin Inhibitors were pooled together for a 0.8 nM total inhibitor concentration. Pools were co-transfected with DharmaFECT Duo into HeLa cells with one of six reporter plasmids specific for each microRNA. Data were normalized to similarly treated empty reporter plasmid only.