miRIDIAN microRNA Mimic Transfection Control with Dy547

Visualize delivery and optimize transfection conditions with a fluorescently labeled mimic control


The miRIDIAN microRNA Mimic Transfection Control is a Dy547-labeled microRNA mimic based on the C. elegans miRNA cel-miR-67 (miRIDIAN Mimic Negative Control #1) for monitoring delivery into human, mouse, and rat cells.
miRIDIAN Transfection Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. For rapid visualization of mimic delivery, utilize the miRIDIAN Mimic Transfection Control with Dy547.

Highlights

  • Non-targeting miRIDIAN Mimic labeled with Dy547 (absorbance/emission max: 557/570 nm)
  • Control designs and modifications are identical to experimental miRIDIAN microRNA Mimics

Applications

  • No identifiable effects on miRNA function in multiple cell lines
  • Transfection can be monitored and visualized by epifluorescence (see Figure 1, Supporting Data)
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
Dye-labeled miRIDIAN Mimic and Hairpin Inhibitor Transfection Controls allow for qualitative evaluation of transfection

Dye-labeled miRIDIAN Mimic and Hairpin Inhibitor Transfection Controls allow for qualitative evaluation of transfection

Dye-labeled miRIDIAN Mimic and Hairpin Inhibitor Transfection Controls allow for qualitative evaluation of transfection

Figure 1. | Images shown are of human (left), rat (middle), and mouse (right) cells 48 hours after transfection with Pierce Dy547-labeled miRIDIAN Mimic Transfection Controls (top panels) and miRIDIAN Hairpin Inhibitor Transfection Controls (bottom panels). Cells were counterstained with Hoechst 33342 (blue, nuclei).