miRIDIAN microRNA Mimic

Up-regulate endogenous microRNA activity for gain-of-function studies


miRIDIAN microRNA Mimics are double-stranded RNA oligonucleotides designed to supplement endogenous microRNA activity. Mimics are available for all human, mouse, and rat microRNAs in the miRBase sequence database.

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miRIDIAN Mimic
C-HMR-XX-0002 2 nmol - $152.00select
miRIDIAN microRNA Mimics are double-stranded RNA oligonucleotides designed to mimic the function of endogenous, mature microRNAs. miRIDIAN Mimics are chemically enhanced with the ON-TARGET modification pattern to preferrentially program RISC with the active microRNA strand. Predesigned mimics are available for all human, mouse, and rat microRNAs in the miRBase Database release 21.0.

Highlights

  • Superior performance in comparison to native, double-stranded microRNA (see Figure 1, Supporting Data)
  • Highly effective mimic of endogenous mature microRNA function (see Figure 2, Supporting Data)
  • Preferential programming of RISC with active strand of microRNA
  • Exclusion of passenger strand through proprietary chemical modification pattern

Applications

  • Supplement microRNA activity to study gain-of-function effects
  • Screen for microRNAs that regulate gene expression and affect cellular pathways
  • Elucidate microRNA involvement in normal biological and disease pathways
  • Identify and validate microRNA targets
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
miRIDIAN microRNA Mimics perform better than native double-stranded microRNA

miRIDIAN microRNA Mimics perform better than native double-stranded microRNA

miRIDIAN microRNA Mimics perform better than native double-stranded microRNA

Figure 1. | miRNA mimic function was assayed in HeLa cells 24 and 48 hours after transfection of 10 nM miR-375 native double-stranded mimic, native double-stranded mimic with modifications, or the miRIDIAN microRNA Mimic using a dual luciferase reporter system and normalized to the control (no mimic).


miRIDIAN microRNA Mimics simulate endogenous mature miRNA function

miRIDIAN microRNA Mimics simulate endogenous mature miRNA function

miRIDIAN microRNA Mimics simulate endogenous mature miRNA function

Figure 2. | miRIDIAN microRNA Mimic function for 11 human miRNAs was assayed in HeLa and HepG2 cells 48 hours after transfection of 10 nM mimic using a dual luciferase reporter system and normalized to the control (no mimic treatment). Similar results were obtained with 1 nM miRIDIAN microRNA Mimic (results not shown).