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Ensure your percent gene editing calculations are correct
The Dharmacon BioIT team has created a beta tool to calculate percent gene editing from a T7EI mismatch detection assay. In a previous featured article, we highlight the importance of properly calculating percent gene editing.
Our BioIT team has provided the research community with a simple calculator to ensure proper calculation of percent gene editing. This calculator should be used in conjunction with the GelAnalyzer 2010a software, which is a freeware application. The GelAnalyzer software makes calculating the band intensity (or volume) much simpler and more robust, since it semi-automatically detects the lanes/bands for each sample in the gel. The resulting band intensities can then be copy/pasted into this beta tool to calculate the percent gene editing found in each sample.
To assist the user in calculating the band intensity using GelAnalyzer 2010a, we also provide a step-by-step tutorial with a few additional troubleshooting steps. Click "Show SOP" to get started.
Check out the tool and let us know what you think by emailing the Bio-IT team.
Author: Jesse Stombaugh, Bioinformatics Developer at Dharmacon
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