• siRNA and transfection reagent calculators

    Estimate the quantities of siRNA/microRNA and DharmaFECT for your high-throughput screening experiments.

    Remember to include consideration for:

    • Investigations in multiple cell lines
    • Pipetting error allowances
    • Reserves for secondary screening
    Typically 20-50 nM. Accell siRNA is 1000 nM (1 µM).
    100 μL is typical working volume in 96 well plate.
    E.g. 30 screens in triplicate, enter 90.
    Typically 0.1-0.5 μL in 96-well.
    E.g. 30 screens in triplicate, enter 90.

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