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This video gives an overview of CRISPR-Cas9 gene editing, followed by the details of how the Edit-R™ CRISPR-Cas9 Gene Engineering Platform simplifies the gene editing workflow by using synthetic RNA. The Edit-R system removes time-consuming steps from the regular gene editing workflow by eliminating the use of sgRNA expression vectors. Instead, a synthetic universal tracrRNA and crRNAs custom designed to target one or more sites in genes of interest are employed. The Edit-R approach results in advantages in speed, simplicity and scalability.