• Video: A demonstration of creating knockins with the Edit-R™ CRISPR-Cas9 system

    The Dharmacon Edit-R CRISPR-Cas9 gene engineering platform has previously been demonstrated to easily create insertions and deletions (indels) to disrupt genes using Cas9, synthetic tracrRNA and a synthetic crRNA. In this poster presentation, we demonstrate that this same system can create precise insertions using the homology-directed repair (HDR) machinery when we supply the cells with a single-strand DNA donor.

    Highlights

    • Demonstration of precise insertions with efficiencies up to 25%
    • Insertion of 10-12 nucleotide sequences with optimized DNA donor oligo homology arm length
    • Simple and straight forward experimental workflow for HDR lipid mediated transfections

    Download the poster for more detailed information

    Homology-directed repair with Edit-R CRISPR-Cas9 and single-strand DNA oligos (PDF 676 KB) »



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