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siRNA-mediated silencing of gene expression has revolutionized the study of biology by enabling rapid and unbiased loss-of-function studies to be carried out in numerous cell types. However, siRNA application in difficult-to-transfect cell types, like primary or suspension cells, is very labor-intensive and technically challenging.
Novel, chemically-modified siRNAs offer a solution to this problem, as these siRNAs enter into difficult-to-transfect cell types without the need of a delivery reagent and are available in many formats, including libraries for RNAi screening. In this webinar we will familiarize you with Dharmacon Accell siRNA and present a study where we have screened an Accell siRNA library targeting the expression of 72 distinct genes in conjunction with a High Content Image Analysis platform as a proof-of-principle strategy to identify genes involved in LFA-1-mediated migration in primary human T cells.
Senior Product Manager – Dharmacon
Louise Baskin is a Senior Product Manager at Dharmacon, having joined the company in 2005. She holds responsibility for all synthetic RNA products, including custom and predesigned siRNA and microRNA reagents.
Senior Research Fellow
Dept. of Clinical Medicine
Trinity College Dublin, Ireland
Dr. Freely’s research focuses on the cell biology of the T cell immune response, particularly the signalling pathways and proteins that regulate T cell activation and migration. He utilises a number of research tools such as siRNAs, screening of RNAi libraries and High Content Analysis to address many of these questions. Characterisation of the pathways that regulate T cell activation and migration holds great promise for modulating T cell immune responses in inflammatory/autoimmune diseases, infection and cancer.