• RNAi success in difficult-to-transfect cells by self-delivering siRNA


    Abstract

    siRNA-mediated silencing of gene expression has revolutionized the study of biology by enabling rapid and unbiased loss-of-function studies to be carried out in numerous cell types. However, siRNA application in difficult-to-transfect cell types, like primary or suspension cells, is very labor-intensive and technically challenging.

    Novel, chemically-modified siRNAs offer a solution to this problem, as these siRNAs enter into difficult-to-transfect cell types without the need of a delivery reagent and are available in many formats, including libraries for RNAi screening. In this webinar we will familiarize you with Dharmacon Accell siRNA and present a study where we have screened an Accell siRNA library targeting the expression of 72 distinct genes in conjunction with a High Content Image Analysis platform as a proof-of-principle strategy to identify genes involved in LFA-1-mediated migration in primary human T cells.

    Presenters

    Presenter: Louise Baskin

    Louise Baskin
    Senior Product Manager – Dharmacon

    Louise Baskin is a Senior Product Manager at Dharmacon, having joined the company in 2005. She holds responsibility for all synthetic RNA products, including custom and predesigned siRNA and microRNA reagents.



    Presenter: Michael Freeley

    Michael Freeley
    Senior Research Fellow
    Dept. of Clinical Medicine
    Trinity College Dublin, Ireland

    Dr. Freely’s research focuses on the cell biology of the T cell immune response, particularly the signalling pathways and proteins that regulate T cell activation and migration. He utilises a number of research tools such as siRNAs, screening of RNAi libraries and High Content Analysis to address many of these questions. Characterisation of the pathways that regulate T cell activation and migration holds great promise for modulating T cell immune responses in inflammatory/autoimmune diseases, infection and cancer.

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