Recommended for dilution of transfection reagent prior to use in Reverse Transfection Format (RTF) siRNA Library plates for resuspension of siRNA.
DharmaFECT Cell Culture Reagent (DCCR) is designed for dilution of DharmaFECT 1
, or 4
Transfection Reagent prior to use in Dharmacon Reverse Transfection format (RTF) siRNA library plates
. The resulting mixture is used to resuspend the pre-plated siRNA, at which time the siRNA:transfection reagent complex is formed. The plates are then ready for cells to be added in the optimal density for siRNA transfection.
Complete resuspension of siRNA in RTF Format
Does not compromise transfection reagent complexes or downstream applications
For resuspension of siRNA provided in tubes or non-RTF library plates, please use 5X siRNA Buffer.
Simple four-step protocol for screening with RTF SMARTpool siRNA Libraries
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