• banner:microRNA - Up-regulate or suppress endogenous mature microRNA function

    microRNA

    Up-regulate or suppress endogenous mature microRNA function with rationally designed synthetic and expressed microRNA modulation reagents.

    Dharmacon microRNA reagents enable short- and long-term modulation of specific microRNAs using a combination of patented technologies. These include chemical and structural enhancements on synthetic reagents and customizable and highly functional design elements on lentiviral reagents.

    Introduction to microRNA modulation

    microRNAs are noncoding RNAs that mediate post-transcriptional gene regulation through a mechanism that involves RISC-mediated binding to complementary sequences largely in the 3’ UTR of mammalian target genes. microRNAs bind to targeted messenger RNAs (mRNAs) via imperfect sequence complementarity resulting largely in mRNA destabilization and translational attenuation. Because microRNA targeting of transcripts is directed by partial sequence complementarity, each microRNA can potentially regulate hundreds of genes.

    Modulation of microRNA levels in both gain- and loss-of function experiments are a fundamental way to understand not only microRNA function, but also the pathways in which they may be involved.

    microRNA mimics

    miRIDIAN microRNA mimics are chemically synthesized synthetic double-stranded RNA molecules which are intended to mimic the endogenous duplex resulting from Dicer processing. One strand represents the mature microRNA as annotated in the miRBase database release 21.0 (www.mirbase.org). Use of the proprietary ON-Target modification ensures that only the desired mature strand is loaded into RISC. miRIDIAN microRNA mimics require delivery into cells using methods similar to those for siRNA (transfection reagents, electroporation, etc.). Individual reagents are available for every microRNA in human, mouse, and rat; and predefined screening libraries are available for human and mouse. Custom rearrays can also be generated and ordered through use of the Cherry-pick Library Plater.

    shMIMIC Lentiviral microRNA and shMIMIC Lentiviral Inducible microRNA constructs are lentiviral vectors which encode native microRNA sequences to achieve exogenously introduced microRNA expression. Based on the patented SMARTvector Lentiviral shRNA platform, they are optimized for highly functional expression and to ensure strand bias so as to only load the specific targeting strand representing the mature microRNA sequence. With the added flexibility of the SMARTchoice promoter and reporter options, constructs can be ordered in numerous configurations ensuing optimal experimental results. Individual constructs are available as high-titer purified lentiviral particles for all human, mouse, and rat microRNAs in the miRbase database release 21.0; pooled screening libraries are also available for human and mouse in both constitutive and inducible formats. Custom pooled screening libraries can also be ordered here.

    microRNA inhibitors

    Introduction of microRNA inhibitors into a biological system results in a loss-of-function assay with a predicted decrease in endogenous microRNA function. miRIDIAN microRNA Hairpin Inhibitors are synthetic single-stranded RNA molecules designed to inhibit endogenous microRNA activity. They are the newest generation proprietary, synthetic inhibitors. The patent-pending molecule combines chemical modifications and completely novel secondary structure motif giving enhanced potency and longevity. This allows for multiplexed microRNA inhibition at very low nanomolar concentrations and with minimal toxicity. Individual reagents are available for every mature microRNA in human, mouse, and rat n the miRBase database release 21.0; and predefined inhibitor screening libraries are available for human and mouse. Custom rearrays can also be created and ordered through use of the Cherry-pick Library Plater.

    • miRIDIAN microRNA Mimics

      Order miRIDIAN microRNA mimics for gain-of-function studies. Use common miRBase identifiers to search for microRNAs in human, mouse and rat.

    • miRIDIAN microRNA Hairpin Inhibitors

      Order microRNA Hairpin Inhibitors to suppress microRNA activity. Use common miRBase identifiers to search for microRNAs in human, mouse and rat.

    • shMIMIC Lentiviral microRNA

      Expressed lentiviral microRNAs with 7 promoter and 3 reporter options for optimal, integrated expression of mature microRNAs in difficult-to-transfect cells.

    • shMIMIC Inducible Lentiviral microRNA

      The industry's most innovative mature microRNA expression system combined with latest generation of powerful Tet-inducible technology.

    • Custom microRNA Modulation Tools

      Modify an existing microRNA mimic or inhibitor, target a novel microRNA, or request an Accell microRNA Inhibitor or Stabilized Mimic for special applications.

    A complete portfolio of positive and negative experimental controls to optimize conditions and design relevant microRNA modulation experiments.

    miRIDIAN Mimic Positive Controls

    miRIDIAN Mimic Negative Controls

    miRIDIAN Mimic Transfection Control Reagents

    miRIDIAN microRNA Hairpin Inhibitor Positive Controls

    miRIDIAN Hairpin Inhibitor Negative Control Reagents

    miRIDIAN Hairpin Inhibitor Transfection Control Reagents

    SMARTvector Controls

    Mimic and Hairpin Inhibitor libraries are available for human, mouse, and rat microRNAs. Provided in 96-well plates for high-throughput phenotypic screening.

    microRNA Resources

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