Use our convenient online tools to order the custom DNA oligo that fits your experimental needs. Whether you are in need of primers for PCR, DNA donor oligos for gene editing using homology-directed repair (HDR), or something in between, we enable your research with custom DNA up to 150 bases in length.
DNA synthesis capabilities supports applications which may require:
- DNA donor oligos for CRISPR-Cas9 HDR
- Primers and templates
- Multiple stabilizing modifications for research specific needs
Custom DNA synthesis of up to 150 bases at either 0.05 nmol or 1.0 µmol scale, with the option of HPLC purification.
Use this online tool to order multiple custom DNA oligos with the same characteristics (scale, purification, 5’ modification)
Gene editing utilizing HDR at the EMX1 locus with multiple DNA donor oligo options
A U2OS integrated Cas9 cell line was plated at 10,000 cells per well (96-well plate) and transfected with synthetic crRNA:tracrRNA and DNA donor oligo using DharmaFECT DUO. HDR insertion efficiency at the EMX1 locus was analyzed 72 hours after transfection. Percent HDR was calculated using an RFLP assay with NheI restriction digestion of EMX1 PCR amplicons.
An in depth explanation of performing HDR with DNA oligo donors
We demonstrate the use of ssDNA oligo donors with synthetic crRNA:tracrRNA and Cas9 nuclease to perform HDR and precisely insert short DNA sequences.
Dharmacon CRISPR-Cas9 tools for targeted genome editing studies. The Edit-R product line includes ready-to-use DNA and RNA components to save time and effort.