banner: Zoonome custom siRNA Libraries - Arrayed RNAi screening libraries for ANY species!

Zoonome Custom siRNA Libraries

On-demand custom siRNA screening libraries for ANY species

Enjoy algorithm-designed siRNA produced with a novel enzymatic method to support large functional genomics studies in non-human or mouse models. Whether you only want to screen 1000 key gene targets or a whole genome, we now have a solution for you! All that's required is species information and a gene list to get a targeted siRNA library for your model organism.

Large scale siRNA screening libraries are no longer limited to human and mouse! For researchers working with alternative species (dog, pig, rat, cow, mosquito, hamster, monkey, etc.), we have developed a novel enzymatic method to generate large arrayed libraries of algorithm-designed siRNAs for ANY species with an annotated genome!*

What are Zoonome siRNA libraries?

  • Utilize a novel enzymatic process for generation of functional, 21 mer siRNA duplexes for arrayed knockdown studies
  • On-demand plated collections of pooled siRNAs (4 algorithm-designed siRNAs in a single well) for RNAi screening studies in non-human or mouse cells
  • Minimum 1000 genes up to whole genome collections for any organism with an annotated genome
  • Expert siRNA design from the Dharmacon SMARTselection algorithm
  • Provided in 384-well plates (96-well upon request) for high-throughput studies

*Target species must have an annotated genome in either the RefSeq or Ensembl™ (Genome Research Limited) database for use as input to the SMARTselection siRNA design algorithm.

Would you like to learn more about Zoonome siRNA Libraries, or submit a request for a price quote? Due to their highly custom nature, all Zoonome libraries are sold by quote only. Complete the form below to have a product expert contact you. Note that orders can take up to 12 weeks to fulfill, so don't delay and inquire today!

The following species have been pre-validated to have an annotated genome database in Ensembl™ (Genome Research Limited) or RefSeq that is suitable for generation of genomewide siRNA designs for creation of a Zoonome siRNA library.

Your species of interest isn't on the list? No problem!

Just submit it, along with the other requested information on the Request Pricing form, and we will determine feasibility for you.

Mammals
Bonobo Pan paniscus
Cat Felis catus
Chimpanzee Pan troglodytes
Chinese Hamster Cricetulus griseus
Cow Bos taurus
Dog Canis familiaris
Dolphin Tursiops truncatus
Elephant Loxodonta africana
Ferret Mustela putorius furo
Goat Capra hircus
Golden Hamster Mesocricetus auratus
Gorilla Gorilla gorilla gorilla
Guinea Pig Cavia porcellus
Horse Equus caballus
Marmoset Callithrix jacchus
Megabat Pteropus vampyrus
Microbat Myotis lucifugus
Olive Baboon Papio anubis
Opossum Monodelphis domestica
Orangutan Pongo pymaeus abelii
Panda Ailuropoda melanoleuca
Pig Sus scrofa
Platypus Ornithorhynchus anatinus
Rabbit Oryctolagus cuniculus
Rat Rattus norvegicus
Rhesus Monkey Macaca mulatta
Rhinoceros Ceratotherium simum simum
Sheep Ovis aries
Sloth Choloepus hoffmanni
Squirrel Ictidomys tridecemlineatus
Wallaby Macropus eugenii
Birds
Chicken Gallus gallus
Duck Anas platyrhynchos
Turkey Meleagris gallopavo
Zebra Finch Taeniopygia guttata
Fish
Fugu Takifugu rubripes
Medaka Oryzias latipes
Nile Tilapia Oreochromis niloticus
Stickleback Gasterosteus aculeatus
Zebrafish Danio rerio
Reptiles
American Alligator Alligator mississippiensis
Chinese Alligator Alligator sinensis
Insects
Armyworm Spodoptera frugiperda
Fruitfly Drosophila melanogaster
Monarch Butterfly Danaus plexippus
Mosquito Aedes aegypti
Amphibians
Frog Xenopus tropicalis
Other
Sea Squirt Ciona intestinalis
Purple Sea Urchin Strongylocentrotus purpuratus
Roundworm Caenorhabditis elegans
Yeast Saccharomyces cerevisiae
workflow: Zoonome alternative methods

Enzymatic methods for siRNA production involve more steps than chemical synthesis, but are less expensive overall and result in a smaller quantity that is appropriate for a single end-user. Zoonome siRNA libraries utilize novel enzymatic methods to generate on-demand arrayed siRNA libraries for model species where synthetic libraries are not available.

Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells

data figure: Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells

Figure 1. CHO-K1 (Chinese hamster) cells were treated with z-siRNA pools targeting 16 genes and assessed for functional knockdown (upper graph) and cell viability (lower graph). All z-siRNA reagents achieved potent knockdown (>75%) while maintaining high cellular viability. Z-siRNAs at 25 nM were delivered with DharmaFECT 4, 0.5 ug per well and assayed 48 hr post-transfection.