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    GIPZ Lentiviral shRNA Libraries

    Perform genome-scale shRNA screening experiments with GIPZ Lentiviral shRNA libraries. Choose a pre-defined gene family library for smaller screening projects.

    Choose from GIPZ whole genome or gene family libraries for human or mouse. Pre-defined libraries have been assembled using innovative bioinformatic tools to reflect the most up-to-date genetic and ontological information. All libraries are offered as glycerol stocks of shRNA clones arrayed in 96-well plates.

    Benefits

    • Designed for increased specificity, less toxicity
    • TurboGFP marks cells expressing shRNA
    • Efficient low copy knockdown
    • Genome-wide human and mouse coverage
    • Perform efficient RNAi in primary and non-dividing cells
    • Guaranteed* knockdown

    *One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used with a functioning positive control. Knockdown should be compared to non-silencing control, and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.

    Bacterial glycerol stock

    Bacterial culture transformed with a plasmid vector, grown in the presence of glycerol for freezer storage and supplied in single tubes (at least three constructs per target gene) and predefined or custom libraries.

    Arrayed library format

    Genome-scale, predefined or custom libraries of bacterial glycerol stocks arrayed in 96-well microtiter plates.

    Gene families and pathways

    Predefined libraries arranged by gene family and/or function and arrayed in 96-well microtiter plates.

    High-titer lentiviral particles

    Transduction-ready concentrated lentiviral particles supplies in tubes. Available as individual lentiviral particles and target gene sets (three constructs per target gene).

    shRNA Starter Kit

    All reagents and protocols necessary for starting a gene silencing experiment are provided in a single, convenient package.

    Fluorescent markers

    Visually track expressed shRNA with TurboGFP (green fluorescent protein; Evrogen, Moscow, Russia) or TurboRFP (red fluorescent protein; Evrogen, Moscow, Russia).

    Which shRNA reagent is best for you?

    Use the table below to assist you in determining the right shRNA reagents for your experimental needs.

    SMARTvector
    shRNA
    GIPZ TRIPZ TRC
    Species Human, Mouse, Rat Human, Mouse Human Human, Mouse
    Promoter Choice of 7 constitutive
    and 4 inducible promoters
    Human CMV Pol II TRE-min-CMV U6 Pol III
    Vector backbone Lentiviral Lentiviral Lentiviral Lentiviral
    Stem-loop format MicroRNA-adapted MicroRNA-adapted MicroRNA-adapted Simple
    Fluorescent reporter gene GFP/RFP GFP RFP
    Guaranteed silencing
    In vivo RNAi
    Create stable cell lines
    Recommended for primary and
    non-dividing cells
    Inducible expression
    Whole genome library availability Human, Mouse, Rat Human, Mouse Human Human
    Formats High-titer lentiviral particles
    Gene families and pathways
    Bacterial glycerol stock
    Arrayed library format
    Gene families and pathways
    High-titer lentiviral particles
    shRNA Starter Kit
    Bacterial glycerol stock
    Arrayed library format
    Gene families and pathways
    shRNA Starter Kit
    Bacterial glycerol stock
    Arrayed library format
    Gene families and pathways

    *For SMARTvector, GIPZ, and TRIPZ lentiviral shRNAs, at least one out of three constructs is guaranteed to reduce target mRNA levels by 70% or more when used in combination with the appropriately matched non-targeting and positive controls.

    Resources for RNAi Screening Libraries

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