Optimal shRNA expression for high-confidence functional analysis results
Our most advanced shRNA platform, SMARTvector Lentiviral shRNAs utilize the flexibility of promoter and reporter options coupled with our most advanced rational design algorithm to target the complete genome for human, mouse, and rat protein-coding and long non-coding genes.
Pooled lentiviral libraries offer an efficient and cost-effective method for screening large numbers of genes without automation. Library construction, pooling techniques, and high-throughput sequencing-compatible screening workflows have been experimentally validated to ensure reproducibility and accurate hit identification. Learn about optimal library design and the pooled screening workflow on our Pooled Lentiviral Screening Libraries page.
Innovative vector design for robust shRNA expression from SMARTvector Lentiviral shRNAs
- SMARTchoice options give you a choice of promoter and reporter (or no reporter!) for optimal performance in your experiment
Rationally designed shRNAs for maximum knockdown and minimal off-targeting
Choose SMARTvector Inducible Lentiviral shRNA for tight regulation of expression from the Tet-On® 3G transactivator protein
Each SMARTvector Lentiviral shRNA Library contains
≥ 5 x 108 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes
8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs
16 x 25 µL (400 µL total) for libraries > 5000 constructs
100 Non-targeting control constructs
272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes.
A data file containing construct sequences, target gene IDs, and counts per millions of mapped reads
SMARTvector Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom SMARTvector Lentiviral shRNA collections are available upon request.
Request a custom collection of human, mouse, or rat gene-targeting shRNAs
Available in pool sizes between 50 to 10,000 constructs
Pools are provided as purified, concentrated lentiviral particles
≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors
Request pricing for a custom pooled library
Products recommended in our validated protocol:
Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required:
SMARTvector Lentiviral shRNA pooled library coverage*:
*Annotations are based on RefSeq v65; coverage and shRNA counts are subject to change without notice
** Clone and gene counts available by request
# targeted genes||
Number pools and average shRNA per pool||
# targeted genes||
Number pools and average shRNA per pool|
16 pools of 9513 shRNA||
18 pools of 9502 shRNA|
6 pools of 9735 shRNA||
8 pools of 9670 shRNA|
1 pool of 3012 shRNA||
1 pool of 3909 shRNA|
1 pool of 2709 shRNA||
1 pool of 2646 shRNA|
1 pool of 1948 shRNA||
1 pool of 2141 shRNA|
1 pool of 3702 shRNA||
1 pool of 4206 shRNA|
1 pool of 5602 shRNA||
1 pool of 5558 shRNA|
1 pool of 4431 shRNA||
1 pool of 4069 shRNA|
Cell Cycle Regulation||
DNA Damage Response||
The SMARTvector Lentiviral shRNA Pooled Libraries are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the containment measures stated below and in applicable laws and regulations are met. Products may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to humans for any purpose or to animals for therapeutic purposes. SMARTvector Lentiviral shRNA Pooled Libraries are provided as lentiviral particles are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious human disease).
Any investigator who purchases lentiviral particle products is responsible for consulting with their institution's health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.