GIPZ Lentiviral shRNA Gene Family and Pathway Libraries

Pre-defined libraries of GIPZ shRNA targeting genes related by function or pathways.


Human and mouse GIPZ shRNA gene family and pathway libraries combine the advantages of microRNA-adapted shRNA with the convenience of pre-defined libraries targeting all genes in a particular gene family or biological pathway and arrayed in 96-well plates.
The GIPZ lentiviral shRNA collection was designed by and built in collaboration with Greg Hannon and Steve Elledge at Cold Spring Harbor Labs. Each shRNA hairpin is cloned into the pGIPZ lentiviral vector and arrayed into 96-well plates to create a renewable RNAi resource.

Highlights

  • Designed for increased specificity, less toxicity
  • TurboGFP marks cells expressing shRNA
  • Efficient low-copy knockdown
  • Genome-wide human and mouse coverage
  • Perform efficient RNAi in primary and non-dividing cells
  • Guaranteed† knockdown

Gene Family and Pathway Libraries

GIPZ human shRNA libraries include:

 
Gene Family # Genes # Constructs
Human Ubiquitin Conjugation Subset 1 88 599
Human Ubiquitin Conjugation Subset 2 113 812
Human Phosphatase 254 1561
Human Ion Channel 347 1884
Human Ubiquitin Conjugation Subset 3 370 2419
Human GPCR 382 2591
Human Protease 478 2559
Human Protein Kinase 709 4675
Human Drug Targets 4753 25,354
Human Druggable Genome 7494 42,454
Human Rest of Genome 10,711 53,249
Human Whole Genome 18,205 95,703

Gene numbers may fluctuate as libraries undergo revision or when gene records are updated.

GIPZ mouse shRNA libraries include: 

 
Gene Family # Genes # Constructs
Mouse Angiogenesis 106 199
Mouse Apoptosis 466 970
Mouse Cell Cycle 532 1117
Mouse Protein Kinases 713 1580
Mouse Transcription Factors 640 1272
Mouse Ubiquitin 96 205
Mouse Ubiquitin Ligase 7494 42,454
Mouse Whole Genome 22,345 101,919

Gene numbers may fluctuate as libraries undergo revision or when gene records are updated.

Footnote

† One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used with a functioning positive control. Knockdown should be compared to non-silencing control, and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third-generation packaging systems, such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

  
HazardousNo
Shelf Life12 Months
Shipping ConditionDry Ice
Storage Condition-80 C
GIPZ lentiviral shRNA gene families and pathway libraries are provided in 96-well microtiter plates containing frozen stock cultures of E. coli in LB (low salt) broth with 8% glycerol, carbenicillin (100 µg/mL) and zeocin (25 µg/mL). We check all cultures for growth prior to shipment.
pGIPZ lentiviral vector

pGIPZ lentiviral vector

pGIPZ lentiviral vector

Figure 1.


Effective knockdown using GIPZ lentiviral shRNA

Effective knockdown using GIPZ lentiviral shRNA

Effective knockdown using GIPZ lentiviral shRNA

Figure 2. OVCAR-8 cells were transduced with GIPZ lentiviral shRNA constructs at MOI = 0.4 to2 in 2 to 4 biological replicates. Cells were puromycin-selected (30 μg/mL) starting 48 hours post-transduction. RNA was isolated 84 hours posttransduction.

qPCR was performed in triplicate via TaqMan® Gene Expression Assays using 18S rRNA as an internal reference. On average, two out of three shRNA produced greater than 70% knockdown compared to the GIPZ Non-targeting Control shRNA.


References

Citations

Product Selection Tools