GIPZ Lentiviral shRNA Libraries

Genome-wide collections for high-throughput shRNA screening


The GIPZ lentiviral shRNA collection was designed by and built in collaboration with Cold Spring Harbor Labs. Each shRNA hairpin is cloned into the pGIPZ lentiviral vector and arrayed into 96-well plates to create a renewable RNAi resource.
GIPZ lentiviral shRNA are provided as individual human or mouse constructs, target gene sets, transfection or transduction starter kits and arrayed libraries. Positive and negative GIPZ shRNA controls are available in glycerol stock and viral particle formats.

Highlights

GIPZ Lentiviral shRNA advantages:

  • Designed for increased specificity, less toxicity
  • TurboGFP marks cells expressing shRNA
  • Efficient low copy knockdown- Important for pooled screens
  • Genome-wide human and mouse coverage
  • Perform efficient RNAi in primary and non-dividing cells
  • Guaranteed* knockdown

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third-generation packaging systems, such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

* One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used with a functioning positive control. Knockdown should be compared to non-silencing control, and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.

NOW AVAILABLE: NEW Human GIPZ Gene Family and Pathway Libraries

Shipping Information

GIPZ lentiviral shRNA libraries are provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100 µg/mL), and zeocin (25 µg/mL). Individual shRNA constructs are shipped as glycerol stock cultures on wet ice. We check all cultures for growth prior to shipment. shRNA clones must be stored at -80°C.

  
HazardousNo
Shipping ConditionDry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C
pGIPZ lentiviral vector

pGIPZ lentiviral vector

pGIPZ lentiviral vector

Figure 1.


Effective knockdown using GIPZ lentiviral shRNA

Effective knockdown using GIPZ lentiviral shRNA

Effective knockdown using GIPZ lentiviral shRNA

Figure 2. OVCAR-8 cells were transduced with GIPZ lentiviral shRNA constructs at MOI = 0.4 to2 in 2 to 4 biological replicates. Cells were puromycin-selected (30 μg/mL) starting 48 hours post-transduction. RNA was isolated 84 hours posttransduction.

qPCR was performed in triplicate via TaqMan® Gene Expression Assays using 18S rRNA as an internal reference. On average, two out of three shRNA produced greater than 70% knockdown compared to the GIPZ Non-targeting Control shRNA.


TurboGFP expression in transduced cells

TurboGFP expression in transduced cells

TurboGFP expression in transduced cells

Figure 3.


References

  1. J. Silva et al., Second-generation shRNA libraries covering the mouse and human genomes. Nat. Genet. 37(11), 1281-8 (2005).
  2. R Boudreau et al., Minimizing variables among hairpin-based RNAi vectors reveals the potency of shRNAs. RNA.14, 1834-1844 (2008).

Citations