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Expert siRNA design provides guaranteed gene silencing from pre-designed genome-wide collections for human, mouse and rat targets.
Simply search for your gene of interest and choose from the available product formats and quantities.
In addition to identifying potent siRNA designs with the SMARTselection algorithm, the creation of siGENOME siRNAs also involves:
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA).
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25nM working concentration.
Dharmacon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
siGENOME SMARTpool reagents demonstrate potency at 5 nM working concentration
A comparison of target gene silencing at 5 and 100 nM concentration illustrates the high potency of siGENOME SMARTpool reagents. 5 or 100 nM siGENOME SMARTpool siRNA reagents targeting the indicated genes was transfected into HeLa cells (10K cells/well) using 0.1 ΜL/well DharmaFECT 1. The negative control (NTC pool) was siGENOME Non-Targeting Pool #2.
Unnecessary sense-strand inactivation can increase off-target activity
Unmodified and sense strand-inactivated siRNAs were used to target five genes. The unmodified siRNAs have natural guide-strand loading characteristics. In four cases off-targets were increased due to enhanced RISC loading of the antisense strand when the sense strand was modified. All siRNAs had comparable silencing potency.
Why not modify ALL siGENOME siRNAs to ensure proper strand loading? It has been demonstrated that forcing antisense (guide) strand entry into RISC may actually INCREASE off-targets due to increased loading of the guide strand and resulting off-target activity by its seed region. siGENOME siRNAs are designed with thermodynamic properties to naturally facilitate guide strand entry to RISC, which has been demonstrated to correlate with functionality. However, in cases where a high-scoring siGENOME siRNA does not possess ideal strand-loading characteristics, a sense (passenger) strand-inhibiting chemical modification (ON-TARGET) is utilized to promote guide strand entry.
Data shown represents genes down-regulated by twofold or more. HEK293 cells were transfected with 100 nM siRNA using 0.2 ΜL of DharmaFECT 1. Data was analyzed at 24 hours by genomewide microarray analysis (Agilent).