The Human siGENOME RTF Ion Channel siRNA Library contains SMARTpool siRNA reagents targeting transmembrane proteins having known or predicted ion channel activity. These include both ligand-gated channels, such as the nicotinic acetylcholine receptors, and voltage-gated channels, such as those that regulate the passage of potassium, sodium, and calcium during polarization and depolarization of the cell membrane.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. They also incorporate rational strand bias with application of ON-TARGET modifications to optimize antisense strand loading into RISC for effective target knockdown.
Patented dual-strand ON-TARGETplus modification pattern on all siRNAs to reduce off-targets
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well plates
Guaranteed target gene knockdown (see Specifications tab)
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Control Catalog No.|
siGENOME Non-targeting siRNA #2||
siGENOME Non-targeting siRNA #3||
siGENOME Non-targeting siRNA #4||
siGENOME Non-targeting siRNA #5||
siGENOME Non-targeting pool #2||
siGENOME Cyclophilin B control pool (H, M, R) Control siRNA||
DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
For a complete list of target genes in this siRNA Library, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.