Lincode GAS5 Control siRNA


A positive control siRNA targeting the GAS5 long noncoding RNA (lncRNA) in human cells. Includes patented dual-strand modifications to minimize off-target effects. Useful for determination of optimal RNAi conditions.
Lincode GAS5 Control siRNA is an effective positive control for RNAi experiments for knockdown of human long, noncoding RNAs (lncRNAs). Growth arrest-specific 5 (GAS5) is expressed during growth arrest phase of the cell cycle. The Lincode GAS5 siRNA carries a proprietary dual-strand modification patterns to have fewer off-targets than unmodified siRNAs. Endogenous GAS5 expression levels should be determined prior to experiments with this reagent.

Highlights

  • Positive control for an endogenous lncRNA in human cells (targets NR_002578)
  • Chemically matched for use with Lincode siRNA reagents
  • Effective silencing verified in multiple cell types

Application considerations

Prior to beginning lncRNA knockdown experiments, the expression level of GAS5, or other target lncRNA, should be assessed in your cell line. If GAS5 levels are not reliably detectable, it is recommended to use ON-TARGETplus GAPD siRNA Control Pool or ON-TARGETplus Cyclophilin B Control Pool as a means of assessing transfection and RNAi efficiency.

  
HazardousNo
Shelf Life12 Months
Shipping ConditionAmbient
Storage Condition-20 C
Cartoon showing Lincode siRNA modifications reducing seed-mediated off-targeting and inactivation of the passenger strand.

Sense strand activity is prevented by siRNA modifications

Cartoon showing Lincode siRNA modifications reducing seed-mediated off-targeting and inactivation of the passenger strand.

Lincode siRNAs are modified with a proprietary dual-strand modification that improves siRNA functionality. Additionally, off-targets are reduced due to:

  • Inactivation of passenger strand activity; driving preferential loading of the guide strand into RISC
  • Novel seed region modifications for disruption of microRNA-like off-targets


Bar chart showing knockdown of GAS5 by siRNA and control pools in HeLa and hNDF cells. Viability remains very close to 100% and knockdowns of greater than 80% are achieved.

Effective silencing of GAS5 by Lincode Control siRNA Reagents

Bar chart showing knockdown of GAS5 by siRNA and control pools in HeLa and hNDF cells. Viability remains very close to 100% and knockdowns of greater than 80% are achieved.

Knockdown of endogenous GAS5 in HeLa cells and hNDF cells by GAS5 Control siRNA and GAS5 Control Pool. All siRNAs were used at 25 nM. Remaining lncRNA was detected by corresponding Solaris qPCR lncRNA expression assay. Knockdown was normalized to Non-targeting control siRNA. Viability was measured by resazurin assay and normalized to Untreated cells.