ON-TARGETplus Non-targeting Control Pool


Negative control pool of four siRNAs designed and microarray tested for minimal targeting of human, mouse or rat genes. ON-TARGETplus modifications reduce potential off-targets. Recommended for determination of baseline cellular responses in RNAi experiments.
ON-TARGETplus Non-targeting Control Pool is the ideal negative control for RNAi experiments in human, mouse, and rat cells. The four component ON-TARGETplus Non-targeting siRNAs were designed to leverage seed-region optimization and patented ON-TARGETplus modification patterns to have fewer off-targets than traditionally designed negative control siRNAs, and screened by genomewide microarray analysis. Changes in mRNA or protein levels in cells treated with these controls reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.

Highlights

  • Ideal negative control for RNAi experiments in human, mouse, and rat cells
  • Chemically matched for use with ON-TARGETplus siRNA reagents
  • Screened by genome-wide microarray analysis for minimal off-targets
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
ON-TARGETplus Non-targeting controls have fewer off-targets, as shown in this microarray heatmap.

ON-TARGETplus Non-targeting controls have fewer off-targets

ON-TARGETplus Non-targeting controls have fewer off-targets, as shown in this microarray heatmap.

RNA samples from HeLa cells were analyzed by microarray expression profiling 24 hours after transfection with the indicated siRNA reagents. Decreases in mRNA levels are shown in green and increases in red. The left panel shows a typical off-target signature of a “Scramble” control siRNA. The right panel shows the off-targets for ON-TARGETplus Non-Targeting Control siRNAs 1 through 4. (D-001810-01, -02, 03, 04).


Citations

  1. P. Baum, K. Fundel-Clemens, S. Kreuz, R. E. Kontermann, A. Weith, D. Mennerich, J. F. Rippmann. Off-Target Analysis of Control siRNA Molecules Reveals Important Differences in the Cytokine Profile and Inflammation Response of Human Fibroblasts. Oligonucleotides. 20(1), 17-25 (2010).