siGLO Cyclophilin B Control siRNA

Correlate fluorescence uptake and silencing activity with a single control


A stable, fluorescent positive control suitable for RNAi experiments in human, mouse, or rat cells. Silences the Cyclophilin B gene and is labeled with DY-547.

siGLO Cyclophilin B Control siRNA is a validated positive control, guaranteed to silence cyclophilin B in human, mouse, or rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.

Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is associated with the secretory pathway. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.

Highlights

  • Targets accession numbers NM_000942 (Human), NM_011149 (Mouse), or NM_022536 (Rat)
  • Labeled with DY-547
  • Absorbance/Emission Max is 557/570 nm
  • A Cy3, Rhodamine or PE filter can be used
  • Significantly extended intensity of the fluorescence signal (7 days or longer)
  • Positive correlation of fluorescence with transfection efficiency and target gene silencing
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

siGLO Cyclophilin B control transfected into HeLa cells (100 nM) with DharmaFECT 1. Images captured 48 hours post-transfection. Cytoplasmic fluorescence is evident in transfected cells.