siGLO Green Transfection Indicator

Provides an unmistakable signal of uptake


A novel FAM-labeled RISC-independent control, modified to localize to the nucleus as a clear signal of transfection success. Useful for determination of optimal siRNA transfection conditions in mammalian cells.

siGLO Green Transfection Indicator is a fluorescent oligonucleotide duplex that localizes to the nucleus, thus concentrating its signal to permit unambiguous visual assessment of uptake into mammalian cells. They are ideal for use in optimization experiments to determine ideal siRNA or microRNA reagent transfection conditions and for monitoring relative delivery efficiency during silencing experiments. siGLO Green (6-FAM) Transfection Indicator exhibits nearly 100% signal localization to the nucleus and represents an ideal choice for lipid-mediated transfection optimization.

Transfection Indicators are intended as a qualitative indicator of delivery and should not be used for quantitative determination of siRNA uptake.

Highlights

  • FAM-labeled Transfection Indicator exhibits nearly 100% signal localization to the nucleus with traditional transfection reagents
  • Absorbance/Emission Max is 494/520 nm, a FITC filter can be used
  • Specially modified, fluorescent RNA duplex for simplified transfection assessment - without performing a functional assay
  • Fluorescent signal localizes to the nucleus as an unmistakable signal of uptake
  • Use as an indicator of transfection success in any RNAi experiment
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
Nuclear localization of fluorescent signal

siGLO Green and siGLO Red Transfection Indicators simplify transfection optimization

Nuclear localization of fluorescent signal

Assessment of nuclear fluorescence permits easy optimization of conditions that result in efficient siRNA delivery into cells. siGLO transfection indicators are specially designed for nuclear localization for a bright, clear signal of transfection success. siGLO Green Transfection Indicator (50 nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 ug/100 uL well). After 24 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).