siGLO Lamin A/C Control siRNA (Rat)

Correlate fluorescence uptake and silencing activity with a single control


A stable, fluorescent positive control suitable for RNAi experiments in rat cells. Silences the rat Lamin A/C gene and is labeled with DY-547.

siGLO Lamin A/C Control siRNA is a validated positive control, guaranteed to silence Lamin A/C in rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.

Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamins A and C have close sequence homology and are derived by differential splicing and alternative polyadenylation from one gene. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.

Highlights

  • Targets accession number: NM_001002016 (Rat)
  • Labeled wtih DY-547
  • Absorbance/Emission Max is 557/570 nm 
  • A Cy3, Rhodamine or PE filter can be used
  • Significantly extended intensity of the fluorescence signal (7 days or longer)
  • Positive correlation of cytoplasmic fluorescence with transfection efficiency
To request a larger quantity or in vivo processing of this control product, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

Cytoplasmic fluorescence upon transfection with siGLO Control siRNA

siGLO Cyclophilin B control transfected into HeLa cells (100 nM) with DharmaFECT 1. Images captured 48 hours post-transfection. Cytoplasmic fluorescence is evident in transfected cells.