Transfection

Transfection

Reagents optimized for your specific application

Dharmacon transfection reagents are designed specifically for small RNA transfection (DharmaFECT), co-transfection of plasmid and small RNA (DharmaFECT Duo), plasmid transfection (DharmaFECT kb) and self delivering siRNA for difficult to transfect cell types (Accell siRNA).

One of the key success factors in any gene modulation experiment (RNAi, overexpression, gene editing) is the introduction of RNA and/or DNA components into your cell line or in vivo system. When applying lipid-based transfection reagents, there are three general measures of an effective transfection reagent:

  • High efficiency delivery
  • Low cellular toxicity
  • Broad dynamic range

We offer a variety of Dharmacon DharmaFECT transfection reagents with demonstrated success for all of these key criteria. Not only do we specialize in transfection of small RNA (siRNA, microRNA, crRNA, tracrRNA) but we offer DharmaFECT Duo, specifically designed for co-delivery of small RNA with plasmid DNA, and DharmaFECT kb for delivery of plasmid DNA.

  • DharmaFECT 1 Transfection Reagent

    The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments. DharmaFECT 1 has been validated in over 35 cell types.

  • DharmaFECT 2 Transfection Reagent

    One of four siRNA/microRNA specific formulations, DharmaFECT 2 is a chemically distinct alternative to one-size-fits-all transfection reagents to achieve high-efficiency silencing in more cell types.

  • DharmaFECT 3 Transfection Reagent

    One of four siRNA/microRNA specific formulations, DharmaFECT 3 is a chemically distinct alternative to one-size-fits-all transfection reagents to achieve high-efficiency silencing in more cell types.

  • DharmaFECT 4 Transfection Reagent

    DharmaFECT 4 Transfection Reagent is chemically distinct from the other DharmaFECT formulations, providing an alternative when DharmaFECT 1 does not achieve optimal results, and is ideal to include in transfection optimization experiments prior to RNAi screening or other high-value experiments

  • DharmaFECT Set of 4 Transfection Reagents

    An aliquot of each of the four DharmaFECT formulations for siRNA/microRNA transfection optimization studies. Useful for determination of the best reagent for your cells and particular experimental conditions.

  • DharmaFECT Duo Transfection Reagent

    For high-confidence co-transfection

  • DharmaFECT kb Transfection Reagent

    Efficient transfection of plasmid DNA with minimal cytotoxicity

  • Accell siRNA Reagents

    Targeted gene silencing in difficult-to-transfect cells

DharmaFECT Transfection Reagent

The table below lists transfection recommendations to help you select the appropriate DharmaFECT formulation for your research. You can also download a PDF version of the DharmaFECT Cell Type Guide here.

All experimental conditions resulted in cell viability and positive control gene silencing of 80% or better.
All experiments were done in 96-well plates with Non-targeting control siRNA and PPIB (Cyclophilin B) or GAPD Control pools at 25 nM; Alamar Blue (viability) and knockdown measured at 24 hours.
Data normalized to untransfected for viability and both untransfected and Non-targeting control for knockdown.
Transfection conditions should always be re-evaluated in the context of a new plate format or assay-specific requirements for cell density.
Human
Cell lineCell typeRecommended DharmaFECT formulationDharmaFECT volume/well (μL) in 96 well platePlating density in 96 well plate (cells per well)Other successful formulations
786-0Kidney adenocarcinoma10.45 × 1032
A549Lung carcinoma10.21 × 1042, 3, 4
BxPC3Pancreas adenocarcinoma20.25 × 1031, 3, 4
DLD-1Colorectal adenocarcinoma20.45 × 1031,3
DU 145Prostate carcinoma10.21 × 1042, 3, 4
NCI-H1299Lung carcinoma20.21 × 1044
HCT-116Colorectal carcinoma20.15 × 1034
HEK293Kidney transformed embryonic cells10.21 × 1042, 4
HeLaCervical epithelial adenocarcinoma10.25 × 1032, 3, 4
HeLa S3Cervical epithelial adenocarcinoma40.45 × 1031, 2, 3
Hep G2Hepatocellular carcinoma40.41 × 1041, 2
hMSCMesenchymal stem cells10.45 × 1032, 3, 4
HT-29Colorectal carcinoma10.25 × 1032, 3, 4
HT1080Fibrosarcoma40.25 × 1031, 2, 3
Huh-7Hepatocarcinoma40.055 × 1031, 2
HUVECUmbilical vein endothelial cells40.22 × 1041, 2
LNCaPProstate carcinoma30.21 × 1041
MCF-10aBreast adenocarcinoma10.21 × 1042
MCF-7Breast adenocarcinoma10.21 × 1042, 4
MDA-MB-453Breast adenocarcinoma20.21 × 1041, 3, 4
MDA-MB-231Breast adenocarcinoma40.15 × 1031
OVCAR-3Ovarian adenocarcinoma10.15 × 1032, 3, 4
PC-3Prostate carcinoma20.21 × 1043
SK-BR3Breast adenocarcinoma20.21 × 1041, 3, 4
SK-OV-3Ovarian adenocarcinoma30.41 × 1041, 2, 4
u87MGBrain glioblastoma10.15 × 1032, 3, 4
Rodent
Cell lineCell typeRecommended DharmaFECT formulationDharmaFECT volume/well (μL) in 96 well platePlating density in 96 well plate (cells per well)Other successful formulations
A7r5Rat aortic smooth muscle20.15 × 1031
C2C12Mouse myoblasts10.25 × 1032, 3, 4
CHO K1Chinese hamster ovary cells40.81 × 1041, 2
ES-D3Mouse embryonic stem cells10.22 × 1032
ES-E14TG2aMouse embryonic stem cells10.22 × 1032
H9c2Rat myoblasts10.21 × 1042, 3, 4
J774A.1Mouse macrophages40.21 × 104-
NIH/3T3Mouse embryonic fibroblasts10.21 × 1043
NRK-49FRat kidney fibroblasts20.21 × 1041, 4
Rat2Rat fibroblasts10.22 × 1042
3T3-L1Mouse embryonic fibroblasts10.25 × 1033
Other
Cell lineCell typeRecommended DharmaFECT formulationDharmaFECT volume/well (μL) in 96 well platePlating density in 96 well plate (cells per well)Other successful formulations
COS-7African green monkey kidney20.45 × 1031, 3, 4

Transfection Resources