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The table below lists transfection recommendations to help you select the appropriate DharmaFECT formulation for your research. You can also download a PDF version of the DharmaFECT Cell Type Guide.
All experimental conditions resulted in cell viability and positive control gene silencing of 80% or better
All experiments were done in 96-well plates with Non-targeting control siRNA and PPIB (Cyclophilin B) or GAPD Control pools at 25 nM; Alamar Blue (viability) and
knockdown measured at 24 hr
Data normalized to untransfected for viability and both untransfected and Non-targeting control for knockdown
Transfection conditions should always be re-evaluated in the context of a new plate format or assay-specific requirements for cell density