• DharmaFECT Cell Type Guide

    Choose DharmaFECT 1, 2, 3, or 4 for optimal siRNA transfection

    The table below lists transfection recommendations to help you select the appropriate DharmaFECT formulation for your research. You can also download a PDF version of the DharmaFECT Cell Type Guide.

    Cell line Cell type Recommended DharmaFECT formulation DharmaFECT volume/well (μl) in 96 well plate Plating density in 96 well plate (cells per well) Other successful formulations
    Human
    786-0 Kidney adenocarcinoma 1 0.4 5 × 103 2
    A549 Lung carcinoma 1 0.2 1 × 104 2, 3, 4
    BxPC3 Pancreas adenocarcinoma 2 0.2 5 × 103 1, 3, 4
    DLD-1 Colorectal adenocarcinoma 2 0.4 5 × 103 1,3
    DU 145 Prostate carcinoma 1 0.2 1 × 104 2, 3, 4
    NCI-H1299 Lung carcinoma 2 0.2 1 × 104 4
    HCT-116 Colorectal carcinoma 2 0.1 5 × 103 4
    HEK293 Kidney transformed embryonic cells 1 0.2 1 × 104 2, 4
    HeLa Cervical epithelial adenocarcinoma 1 0.2 5 × 103 2, 3, 4
    HeLa S3 Cervical epithelial adenocarcinoma 4 0.4 5 × 103 1, 2, 3
    Hep G2 Hepatocellular carcinoma 4 0.4 1 × 104 1, 2
    hMSC Mesenchymal stem cells 1 0.4 5 × 103 2, 3, 4
    HT-29 Colorectal carcinoma 1 0.2 5 × 103 2, 3, 4
    HT1080 Fibrosarcoma 4 0.2 5 × 103 1, 2, 3
    Huh-7 Hepatocarcinoma 4 0.05 5 × 103 1, 2
    HUVEC Umbilical vein endothelial cells 4 0.2 2 × 104 1, 2
    LNCaP Prostate carcinoma 3 0.2 1 × 104 1
    MCF-10a Breast adenocarcinoma 1 0.2 1 × 104 2
    MCF-7 Breast adenocarcinoma 1 0.2 1 × 104 2, 4
    MDA-MB-453 Breast adenocarcinoma 2 0.2 1 × 104 1, 3, 4
    MDA-MB-231 Breast adenocarcinoma 4 0.1 5 × 103 1
    OVCAR-3 Ovarian adenocarcinoma 1 0.1 5 × 103 2, 3, 4
    PC-3 Prostate carcinoma 2 0.2 1 × 104 3
    SK-BR3 Breast adenocarcinoma 2 0.2 1 × 104 1, 3, 4
    SK-OV-3 Ovarian adenocarcinoma 3 0.4 1 × 104 1, 2, 4
    u87MG Brain glioblastoma 1 0.1 5 × 103 2, 3, 4
    Rodent
    A7r5 Rat aortic smooth muscle 2 0.1 5 × 103 1
    C2C12 Mouse myoblasts 1 0.2 5 × 103 2, 3, 4
    CHO K1 Chinese hamster ovary cells 4 0.8 1 × 104 1, 2
    ES-D3 Mouse embryonic stem cells 1 0.2 2 × 103 2
    ES-E14TG2a Mouse embryonic stem cells 1 0.2 2 × 103 2
    H9c2 Rat myoblasts 1 0.2 1 × 104 2, 3, 4
    J774A.1 Mouse macrophages 4 0.2 1 × 104 -
    NIH/3T3 Mouse embryonic fibroblasts 1 0.2 1 × 104 3
    NRK-49F Rat kidney fibroblasts 2 0.2 1 × 104 1, 4
    Rat2 Rat fibroblasts 1 0.2 2 × 104 2
    3T3-L1 Mouse embryonic fibroblasts 1 0.2 5 × 103 3
    Other
    COS-7 African green monkey kidney 2 0.4 5 × 103 1, 3, 4

    All experimental conditions resulted in cell viability and positive control gene silencing of 80% or better
    All experiments were done in 96-well plates with Non-targeting control siRNA and PPIB (Cyclophilin B) or GAPD Control pools at 25 nM; Alamar Blue (viability) and knockdown measured at 24 hr
    Data normalized to untransfected for viability and both untransfected and Non-targeting control for knockdown
    Transfection conditions should always be re-evaluated in the context of a new plate format or assay-specific requirements for cell density

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